Source:http://linkedlifedata.com/resource/pubmed/id/12744707
Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
|
| pubmed:dateCreated |
2003-5-14
|
| pubmed:abstractText |
Sugar phosphate backbone conformations are a structural element inextricably involved in a complete understanding of specific recognition nucleic acid ligand interactions, from early stage discrimination of the correct target to complexation per se, including any structural adaptation on binding. The collective results of high resolution DNA, RNA and protein/DNA crystal structures provide an opportunity for an improved and enhanced statistical analysis of standard and unusual sugar-phosphate backbone conformations together with corresponding dinucleotide sequence effects as a basis for further exploration of conformational effects on binding. In this study, we have analyzed the conformations of all relevant crystal structures in the nucleic acids data base, determined the frequency distribution of all possible epsilon, zeta, alpha, beta and gamma backbone angle arrangements within four nucleic acid categories (A-RNA and A-DNA, free and bound B-DNA) and explored the relationships between backbone angles, sugar puckers and selected helical parameters. The trends in the correlations are found to be similar regardless of the nucleic acid category. It is interesting that specific structural effects exhibited by the different unusual backbone sub-states are in some cases contravariant. Certain alpha/gamma changes are accompanied by C3' endo (north) sugars, small twist angles and positive values of base pair roll, and favor a displacement of nucleotide bases towards the minor groove compared to that of canonical B form structures. Unusual epsilon/zeta combinations occur with C2' (south) sugars, high twist angles, negative values of base pair roll, and base displacements towards the major groove. Furthermore, any unusual backbone correlates with a reduced dispersion of equilibrium structural parameters of the whole double helix, as evidenced by the reduced standard deviations of almost all conformational parameters. Finally, a strong sequence effect is displayed in the free oligomers, but reduced somewhat in the ligand bound forms. The most variable steps are GpA and CpA, and, to a lesser extent, their partners TpC and TpG. The results provide a basis for considering if the variable and non-variable steps within a biological active sequence precisely determine morphological structural features as the curvature direction, the groove depth, and the accessibility of base pair for non covalent associations.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jun
|
| pubmed:issn |
0739-1102
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
20
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
771-88
|
| pubmed:meshHeading |
pubmed-meshheading:12744707-Carbohydrate Conformation,
pubmed-meshheading:12744707-Carbohydrate Sequence,
pubmed-meshheading:12744707-Carbohydrates,
pubmed-meshheading:12744707-Crystallography, X-Ray,
pubmed-meshheading:12744707-DNA-Binding Proteins,
pubmed-meshheading:12744707-Molecular Sequence Data,
pubmed-meshheading:12744707-Nucleic Acid Conformation,
pubmed-meshheading:12744707-Oligonucleotides
|
| pubmed:year |
2003
|
| pubmed:articleTitle |
Conformational characteristics and correlations in crystal structures of nucleic acid oligonucleotides: evidence for sub-states.
|
| pubmed:affiliation |
Laboratoire de Biochimie Theorique, CNRS UPR 9080, Institut de Biologie Physico-chimique, 13 rue P. et M. Curie, Paris 75005, France. Dragana.Djuranovic@ibpc.fr
|
| pubmed:publicationType |
Journal Article
|