Linked Life Data

12740013

Source:http://linkedlifedata.com/resource/pubmed/id/12740013

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2003-5-12
pubmed:abstractText
The aim of this work was to study the possible relationship between pancreatic duodenal homeobox-1 (Pdx-1) and islet neogenesis-associated protein (INGAP) during induced islet neogenesis. Pregnant hamsters were fed with (S) and without (C) sucrose, and glycemia, insulin secretion in vitro, and pancreas immunomorphometric parameters were measured in their 7-day-old offspring. S offspring had significantly lower glycemic levels than C animals. Insulin release in response to increasing glucose concentrations in the incubation medium (2-16 mM glucose) did not increase in pancreata from either C or S offspring. However, pancreata from S offspring released more insulin than those from C animals. In S offspring, beta-cell mass, beta-cell replication rate and islet neogenesis increased significantly, with a simultaneous decrease in beta-cell apoptotic rate. INGAP- and Pdx-1-positive cell mass also increased in the islets and among acinar and duct cells. We found two subpopulations of Pdx-1 cells: INGAP-positive and INGAP-negative. Pdx-1/INGAP-positive cells did not stain with insulin, glucagon, somatostatin, pancreatic polypeptide, or neurogenin 3 antibodies. The increment of Pdx-1/INGAP-positive cells represented the major contribution to the Pdx-1 cell mass increase. Such increments varied among pancreas subsectors: ductal>insular>extrainsular. Our results suggested that INGAP participates in the regulation of islet neogenesis, and Pdx-1/INGAP-positive cells represent a new stem cell subpopulation at an early stage of development, highly activateable in neogenesis.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0022-0795
pubmed:author
pubmed:issnType
Print
pubmed:volume
177
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
249-59
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:12740013-Animals, pubmed-meshheading:12740013-Animals, Newborn, pubmed-meshheading:12740013-Antigens, Neoplasm, pubmed-meshheading:12740013-Apoptosis, pubmed-meshheading:12740013-Biological Markers, pubmed-meshheading:12740013-Body Weight, pubmed-meshheading:12740013-Cricetinae, pubmed-meshheading:12740013-Female, pubmed-meshheading:12740013-Homeodomain Proteins, pubmed-meshheading:12740013-Immunohistochemistry, pubmed-meshheading:12740013-Insulin, pubmed-meshheading:12740013-Islets of Langerhans, pubmed-meshheading:12740013-Lectins, C-Type, pubmed-meshheading:12740013-Pancreas, pubmed-meshheading:12740013-Pregnancy, pubmed-meshheading:12740013-Proteins, pubmed-meshheading:12740013-Stem Cells, pubmed-meshheading:12740013-Trans-Activators, pubmed-meshheading:12740013-Tumor Markers, Biological
pubmed:year
2003
pubmed:articleTitle
Pancreatic duodenal homeobox-1 and islet neogenesis-associated protein: a possible combined marker of activateable pancreatic cell precursors.
pubmed:affiliation
CENEXA - Centre of Experimental and Applied Endocrinology (UNLP-CONICET, PAHO/WHO Collaborating Centre), National University of La Plata School of Medicine, La Plata, Argentina.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't