Linked Life Data

12739009

Source:http://linkedlifedata.com/resource/pubmed/id/12739009

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2003-5-9
pubmed:abstractText
The Noble (Nb) rat model has been used in the study of hormonal carcinogenesis of mammary and prostate glands, as this rat strain is susceptible to tumor induction in these glands by hormonal treatments. Recently, we demonstrated that this rat strain can develop spontaneously mammary tumors at high incidence in aged animals and also show high sensitivity to chemical carcinogens (DMBA and MNU) and combined treatments with sex hormones in mammary tumor induction. In the present study, we examined and compared the expression of hormone receptors [including estrogen receptors (ERalpha and ERbeta), androgen receptor (AR), progesterone receptor (PR), prolactin receptor (PRLR)] and prolactin (PRL) by immunohistochemistry, Western blotting and RT-PCR in spontaneous mammary tumors, and mammary tumors induced by sex hormones (T+E2 and T+DES for 8-10 months) and DMBA in Nb rat model. Immunohistochemistry and Western blotting showed that both the spontaneously developed and hormone-induced carcinomas exhibited strong immunoreactivity of ERalpha, ERbeta, AR, PR and PRLR, while the spontaneous fibroadenomas showed weak to moderate immunoreactivity of ERalpha and PRLR, whereas the DMBA-induced carcinomas exhibited weak to moderate immunoreactivity of ERalpha, AR, PR and PRLR, and sporadic weak ERbeta immunoreactivity. RT-PCR analyses showed that mRNA expression pattern of these markers resembled that of proteins. In addition, weak mRNA expression of PRL was detected in spontaneous carcinomas and carcinomas induced by DMBA and hormones, suggesting that PRL could be produced locally within the tumors. The results showed that the expression status of hormone receptors and PRL was different in spontaneous mammary tumors and tumors induced by carcinogen or hormones, suggesting that the extent of involvement of steroid hormones and their receptors in the spontaneous, carcinogen- or hormone-induced mammary carcinogenesis might be different.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1019-6439
pubmed:author
pubmed:issnType
Print
pubmed:volume
22
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1383-95
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:12739009-9,10-Dimethyl-1,2-benzanthracene, pubmed-meshheading:12739009-Animals, pubmed-meshheading:12739009-Base Sequence, pubmed-meshheading:12739009-Carcinogens, pubmed-meshheading:12739009-DNA Primers, pubmed-meshheading:12739009-Female, pubmed-meshheading:12739009-Hormones, pubmed-meshheading:12739009-Immunohistochemistry, pubmed-meshheading:12739009-Mammary Neoplasms, Animal, pubmed-meshheading:12739009-Mammary Neoplasms, Experimental, pubmed-meshheading:12739009-Methylnitrosourea, pubmed-meshheading:12739009-Polymerase Chain Reaction, pubmed-meshheading:12739009-Rats, pubmed-meshheading:12739009-Rats, Inbred Strains, pubmed-meshheading:12739009-Receptors, Estrogen, pubmed-meshheading:12739009-Receptors, Progesterone, pubmed-meshheading:12739009-Receptors, Prolactin
pubmed:year
2003
pubmed:articleTitle
An expression study of hormone receptors in spontaneously developed, carcinogen-induced and hormone-induced mammary tumors in female Noble rats.
pubmed:affiliation
Department of Anatomy, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't