Source:http://linkedlifedata.com/resource/pubmed/id/12738765
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
30
|
| pubmed:dateCreated |
2003-7-21
|
| pubmed:databankReference | |
| pubmed:abstractText |
RbsD is the only protein whose biochemical function is unknown among the six gene products of the rbs operon involved in the active transport of ribose. FucU, a paralogue of RbsD conserved from bacteria to human, is also the only protein whose function is unknown among the seven gene products of the l-fucose regulon. Here we report the crystal structures of Bacillus subtilis RbsD, which reveals a novel decameric toroidal assembly of the protein. Nuclear magnetic resonance and other studies on RbsD reveal that the intersubunit cleft of the protein binds specific forms of d-ribose, but it does not have an enzyme activity toward the sugar. Likewise, FucU binds l-fucose but lacks an enzyme activity toward this sugar. We conclude that RbsD and FucU are cytoplasmic sugar-binding proteins, a novel class of proteins whose functional role may lie in helping influx of the sugar substrates.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jul
|
| pubmed:issn |
0021-9258
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
25
|
| pubmed:volume |
278
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
28173-80
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:12738765-Amino Acid Sequence,
pubmed-meshheading:12738765-Bacillus subtilis,
pubmed-meshheading:12738765-Binding Sites,
pubmed-meshheading:12738765-Calorimetry,
pubmed-meshheading:12738765-Crystallography, X-Ray,
pubmed-meshheading:12738765-Cytoplasm,
pubmed-meshheading:12738765-Escherichia coli,
pubmed-meshheading:12738765-Fucose,
pubmed-meshheading:12738765-Glycerol,
pubmed-meshheading:12738765-Magnetic Resonance Spectroscopy,
pubmed-meshheading:12738765-Models, Molecular,
pubmed-meshheading:12738765-Molecular Sequence Data,
pubmed-meshheading:12738765-Monosaccharide Transport Proteins,
pubmed-meshheading:12738765-Polymerase Chain Reaction,
pubmed-meshheading:12738765-Protein Binding,
pubmed-meshheading:12738765-Protein Folding,
pubmed-meshheading:12738765-Protein Structure, Secondary,
pubmed-meshheading:12738765-Ribose,
pubmed-meshheading:12738765-Sequence Homology, Amino Acid
|
| pubmed:year |
2003
|
| pubmed:articleTitle |
Crystal structures of RbsD leading to the identification of cytoplasmic sugar-binding proteins with a novel folding architecture.
|
| pubmed:affiliation |
Center for Biomolecular Recognition and Division of Molecular and Life Science, Department of Life Science, Pohang University of Science and Technology, Pohang, Kyungbuk, 790-784, Korea.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|