Source:http://linkedlifedata.com/resource/pubmed/id/12736145
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
6
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pubmed:dateCreated |
2003-5-8
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pubmed:abstractText |
Penetration of the gut epithelial barrier by intact luminal antigen is necessary for immunologically mediated pathophysiology in the context of food allergy. We investigated if glucagon-like peptide-2 (GLP-2) could affect immediate hypersensitivity and late-phase allergic inflammation in a murine model. Mice were sensitized to horseradish peroxidase (HRP); studies were conducted 14 days later. Mice were treated with 5 microg GLP-2 subcutaneously 4 h before antigen challenge. For immediate hypersensitivity, jejunal segments in Ussing chambers were challenged by luminal HRP antigen. GLP-2 treatment reduced the uptake of HRP and the antigen-induced secretory response after luminal challenge. GLP-2 appears to reduce macromolecular uptake independent of the CD23-mediated enhanced antigen uptake pathway. For the late phase, mice were gavaged with antigen, and 48 h later the function and histology of the jejunum were examined. GLP-2 prevented the usual prolonged permeability defect and reduced the number of inflammatory cells in the mucosa. Our studies demonstrate that a single treatment of sensitized mice with GLP diminishes both immediate and late-phase hypersensitivity reactions characteristic of food allergy by inhibiting transepithelial uptake of antigen.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0193-1857
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
284
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
G905-12
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:12736145-Animals,
pubmed-meshheading:12736145-Cell Membrane Permeability,
pubmed-meshheading:12736145-Disease Models, Animal,
pubmed-meshheading:12736145-Endosomes,
pubmed-meshheading:12736145-Food Hypersensitivity,
pubmed-meshheading:12736145-Glucagon-Like Peptide 2,
pubmed-meshheading:12736145-Glucagon-Like Peptides,
pubmed-meshheading:12736145-Horseradish Peroxidase,
pubmed-meshheading:12736145-Humans,
pubmed-meshheading:12736145-Jejunum,
pubmed-meshheading:12736145-Male,
pubmed-meshheading:12736145-Mice,
pubmed-meshheading:12736145-Mice, Inbred BALB C,
pubmed-meshheading:12736145-Peptides
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pubmed:year |
2003
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pubmed:articleTitle |
Glucagon-like peptide-2-enhanced barrier function reduces pathophysiology in a model of food allergy.
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pubmed:affiliation |
Intestinal Disease Research Programme, McMaster University, Hamilton, Ontario, Canada L8N 3Z5.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
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