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rdf:type |
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lifeskim:mentions |
umls-concept:C0033684,
umls-concept:C0039082,
umls-concept:C0215407,
umls-concept:C0439855,
umls-concept:C0599851,
umls-concept:C0676792,
umls-concept:C1412171,
umls-concept:C1412400,
umls-concept:C1421482,
umls-concept:C1658596,
umls-concept:C1704675,
umls-concept:C1705294,
umls-concept:C2700384
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pubmed:issue |
28
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pubmed:dateCreated |
2003-7-4
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pubmed:abstractText |
The WASP and cortactin families constitute two distinct classes of Arp2/3 modulators in mammalian cells. Physical and functional interactions among the Arp2/3 complex, VCA (a functional domain of N-WASP), and cortactin were examined under conditions that were with or without actin polymerization. In the absence of actin, cortactin binds significantly weaker to the Arp2/3 complex than VCA. At concentrations of VCA 20-fold lower than cortactin, the association of cortactin with the Arp2/3 complex was nearly abolished. Analysis of the cells infected with Shigella demonstrated that N-WASP located at the tip of the bacterium, whereas cortactin accumulated in the comet tail. Interestingly, cortactin promotes Arp2/3 complex-mediated actin polymerization and actin branching in the presence of VCA at a saturating concentration, and cortactin acquired 20 nm affinity for the Arp2/3 complex during actin polymerization. The interaction of VCA with the Arp2/3 complex was reduced in the presence of both cortactin and actin. Moreover, VCA reduced its affinity for Arp2/3 complex at branching sites that were stabilized by phalloidin. These data imply a novel mechanism for the de novo assembly of a branched actin network that involves a coordinated sequential interaction of N-WASP and cortactin with the Arp2/3 complex.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/ACTR2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/ACTR3 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Actin-Related Protein 2,
http://linkedlifedata.com/resource/pubmed/chemical/Actin-Related Protein 3,
http://linkedlifedata.com/resource/pubmed/chemical/Actins,
http://linkedlifedata.com/resource/pubmed/chemical/Actr2 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Actr3 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/CTTN protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Cortactin,
http://linkedlifedata.com/resource/pubmed/chemical/Cttn protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Cytoskeletal Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Glutathione Transferase,
http://linkedlifedata.com/resource/pubmed/chemical/Microfilament Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Tissue Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/WASL protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Wasl protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Wiskott-Aldrich Syndrome Protein...
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
11
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pubmed:volume |
278
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
26086-93
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:12732638-Actin-Related Protein 2,
pubmed-meshheading:12732638-Actin-Related Protein 3,
pubmed-meshheading:12732638-Actins,
pubmed-meshheading:12732638-Animals,
pubmed-meshheading:12732638-Cortactin,
pubmed-meshheading:12732638-Cytoskeletal Proteins,
pubmed-meshheading:12732638-Dose-Response Relationship, Drug,
pubmed-meshheading:12732638-Glutathione Transferase,
pubmed-meshheading:12732638-Humans,
pubmed-meshheading:12732638-Mice,
pubmed-meshheading:12732638-Microfilament Proteins,
pubmed-meshheading:12732638-Microscopy, Fluorescence,
pubmed-meshheading:12732638-Models, Biological,
pubmed-meshheading:12732638-Muscle, Skeletal,
pubmed-meshheading:12732638-Nerve Tissue Proteins,
pubmed-meshheading:12732638-Protein Binding,
pubmed-meshheading:12732638-Protein Structure, Tertiary,
pubmed-meshheading:12732638-Rabbits,
pubmed-meshheading:12732638-Time Factors,
pubmed-meshheading:12732638-Wiskott-Aldrich Syndrome Protein, Neuronal
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pubmed:year |
2003
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pubmed:articleTitle |
Sequential interaction of actin-related proteins 2 and 3 (Arp2/3) complex with neural Wiscott-Aldrich syndrome protein (N-WASP) and cortactin during branched actin filament network formation.
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pubmed:affiliation |
Department of Experimental Pathology, Jerome H. Holland Laboratory for the Biomedical Sciences, American Red Cross, Rockville, Maryland 20855, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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