Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2003-5-6
pubmed:databankReference
pubmed:abstractText
Rhodococcus (opacus) erythropolis HL PM-1 grows on 2,4,6-trinitrophenol or 2,4-dinitrophenol (2,4-DNP) as a sole nitrogen source. The NADPH-dependent F(420) reductase (NDFR; encoded by npdG) and the hydride transferase II (HTII; encoded by npdI) of the strain were previously shown to convert both nitrophenols to their respective hydride Meisenheimer complexes. In the present study, npdG and npdI were amplified from six 2,4-DNP degrading Rhodococcus spp. The genes showed sequence similarities of 86 to 99% to the respective npd genes of strain HL PM-1. Heterologous expression of the npdG and npdI genes showed that they were involved in 2,4-DNP degradation. Sequence analyses of both the NDFRs and the HTIIs revealed conserved domains which may be involved in binding of NADPH or F(420). Phylogenetic analyses of the NDFRs showed that they represent a new group in the family of F(420)-dependent NADPH reductases. Phylogenetic analyses of the HTIIs revealed that they form an additional group in the family of F(420)-dependent glucose-6-phosphate dehydrogenases and F(420)-dependent N(5),N(10)-methylenetetrahydromethanopterin reductases. Thus, the NDFRs and the HTIIs may each represent a novel group of F(420)-dependent enzymes involved in catabolism.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/12732545-10103224, http://linkedlifedata.com/resource/pubmed/commentcorrection/12732545-10217752, http://linkedlifedata.com/resource/pubmed/commentcorrection/12732545-10339815, http://linkedlifedata.com/resource/pubmed/commentcorrection/12732545-11515368, http://linkedlifedata.com/resource/pubmed/commentcorrection/12732545-11882715, http://linkedlifedata.com/resource/pubmed/commentcorrection/12732545-1444407, http://linkedlifedata.com/resource/pubmed/commentcorrection/12732545-1444408, http://linkedlifedata.com/resource/pubmed/commentcorrection/12732545-1645124, http://linkedlifedata.com/resource/pubmed/commentcorrection/12732545-2231712, http://linkedlifedata.com/resource/pubmed/commentcorrection/12732545-2265755, http://linkedlifedata.com/resource/pubmed/commentcorrection/12732545-2492438, http://linkedlifedata.com/resource/pubmed/commentcorrection/12732545-3422704, http://linkedlifedata.com/resource/pubmed/commentcorrection/12732545-4852581, http://linkedlifedata.com/resource/pubmed/commentcorrection/12732545-7984417, http://linkedlifedata.com/resource/pubmed/commentcorrection/12732545-8085840, http://linkedlifedata.com/resource/pubmed/commentcorrection/12732545-8250573, http://linkedlifedata.com/resource/pubmed/commentcorrection/12732545-8360625, http://linkedlifedata.com/resource/pubmed/commentcorrection/12732545-8702398, http://linkedlifedata.com/resource/pubmed/commentcorrection/12732545-8757942, http://linkedlifedata.com/resource/pubmed/commentcorrection/12732545-8990288, http://linkedlifedata.com/resource/pubmed/commentcorrection/12732545-8997717, http://linkedlifedata.com/resource/pubmed/commentcorrection/12732545-9325428, http://linkedlifedata.com/resource/pubmed/commentcorrection/12732545-9381173, http://linkedlifedata.com/resource/pubmed/commentcorrection/12732545-9390451, http://linkedlifedata.com/resource/pubmed/commentcorrection/12732545-9555906, http://linkedlifedata.com/resource/pubmed/commentcorrection/12732545-9864324
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0099-2240
pubmed:author
pubmed:issnType
Print
pubmed:volume
69
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2748-54
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
Homologous npdGI genes in 2,4-dinitrophenol- and 4-nitrophenol-degrading Rhodococcus spp.
pubmed:affiliation
Institute of Microbiology, University of Stuttgart, Allmandring 31, 70550 Stuttgart, Germany. gesche.heiss@po.uni-stuttgart.de
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't