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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
28
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pubmed:dateCreated |
2003-7-4
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pubmed:abstractText |
A putative oncogene bcl-3 was originally identified and cloned at the breakpoint in the recurring chromosome translocation t(14;19) found in some cases of B cell chronic lymphocytic leukemia. Studies of bcl-3-deficient mice demonstrated a critical role for bcl-3 in the development of a normal immune response and the formation of germinal centers in secondary lymphoid organs. However, the molecular mechanism that underlies B cell leukemogenesis and the knockout mouse phenotype remains unclear. Here we have identified and characterized BCL-3-binding protein (B3BP) as a protein interacting specifically with the bcl-3 gene product (BCL-3) by a yeast two-hybrid screen. We found that B3BP associates with not only BCL-3 but also p300/CBP histone acetyltransferases. The N-terminal region of B3BP that contains the ATP-binding site is important for the interaction with BCL-3 and p300/CBP. Homology searches indicate that the ATP-binding region of B3BP, which contains a typical Walker-type ATP-binding P-loop, most resembles that of 2',3'-cyclic nucleotide 3'-phosphodiesterase of mammals and polynucleotide kinase of T4 bacteriophage. In fact B3BP shows intrinsic ATP binding and hydrolyzing activity. Furthermore, we demonstrated that B3BP is a 5'-polynucleotide kinase. We also found a small MutS-related domain, which is thought to be involved in the DNA repair or recombination reaction, in the C-terminal region of B3BP, and it shows nicking endonuclease activity. These observations might help to gain new insights into the function of BCL-3 and p300/CBP, especially the coupling of transcription with repair or recombination.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adenosine Triphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/E1A-Associated p300 Protein,
http://linkedlifedata.com/resource/pubmed/chemical/Ep300 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Glutathione Transferase,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Polynucleotide 5'-Hydroxyl-Kinase,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/proto-oncogene protein bcl-3
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
11
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pubmed:volume |
278
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
26102-10
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:12730195-Adenosine Triphosphate,
pubmed-meshheading:12730195-Amino Acid Sequence,
pubmed-meshheading:12730195-Animals,
pubmed-meshheading:12730195-B-Lymphocytes,
pubmed-meshheading:12730195-Bacteriophage T4,
pubmed-meshheading:12730195-Binding Sites,
pubmed-meshheading:12730195-Blotting, Western,
pubmed-meshheading:12730195-Carrier Proteins,
pubmed-meshheading:12730195-Cell Line,
pubmed-meshheading:12730195-DNA, Complementary,
pubmed-meshheading:12730195-DNA Repair,
pubmed-meshheading:12730195-E1A-Associated p300 Protein,
pubmed-meshheading:12730195-Genetic Vectors,
pubmed-meshheading:12730195-Glutathione Transferase,
pubmed-meshheading:12730195-Humans,
pubmed-meshheading:12730195-Hydrolysis,
pubmed-meshheading:12730195-Mice,
pubmed-meshheading:12730195-Mice, Knockout,
pubmed-meshheading:12730195-Models, Genetic,
pubmed-meshheading:12730195-Molecular Sequence Data,
pubmed-meshheading:12730195-Nuclear Proteins,
pubmed-meshheading:12730195-Plasmids,
pubmed-meshheading:12730195-Polynucleotide 5'-Hydroxyl-Kinase,
pubmed-meshheading:12730195-Precipitin Tests,
pubmed-meshheading:12730195-Protein Binding,
pubmed-meshheading:12730195-Protein Structure, Tertiary,
pubmed-meshheading:12730195-Proto-Oncogene Proteins,
pubmed-meshheading:12730195-Recombinant Proteins,
pubmed-meshheading:12730195-Recombination, Genetic,
pubmed-meshheading:12730195-Sequence Homology, Amino Acid,
pubmed-meshheading:12730195-Time Factors,
pubmed-meshheading:12730195-Trans-Activators,
pubmed-meshheading:12730195-Transcription, Genetic,
pubmed-meshheading:12730195-Transcription Factors,
pubmed-meshheading:12730195-Transfection,
pubmed-meshheading:12730195-Two-Hybrid System Techniques
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pubmed:year |
2003
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pubmed:articleTitle |
Identification and characterization of BCL-3-binding protein: implications for transcription and DNA repair or recombination.
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pubmed:affiliation |
Department of Tumor Cell Biology, The Tokyo Metropolitan Institute of Medical Science, 3-18-22 Honkomagome, Bunkyo-ku, Tokyo 113-8613, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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