Source:http://linkedlifedata.com/resource/pubmed/id/12730057
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
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| pubmed:dateCreated |
2003-8-13
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| pubmed:abstractText |
To examine the receptor specificity and the mechanism of opioid peptide-induced protection, we examined freshly isolated adult rabbit cardiomyocytes subjected to simulated ischemia. Cell death as a function of time was assessed by trypan blue permeability. Dynorphin B (DynB) and Met5-enkephalin (ME) limitation of cell death (expressed as area under the curve) was sensitive to blockade by naltrindole (NTI, a delta-selective antagonist) and 5'-guanidinyl-17-(cyclopropylmethyl)-6,7-dehydro-4,5alpha-epoxy-3,14-dihydroxy-6,7-2',3'-indolomorphinan (GNTI dihydrochloride, a kappa-selective antagonist): 85.7 +/- 2.7 and 142.9 +/- 2.7 with DynB and DynB + NTI, respectively (P < 0.001), 94.1 +/- 4.2 and 164.5 +/- 7.3 with DynB and DynB + GNTI, respectively (P < 0.001), 111.9 +/- 7.0 and 192.1 +/- 6.4 with ME and ME + NTI, respectively (P < 0.001), and 120.2 +/- 4.3 and 170.0 +/- 3.3 with ME and ME + GNTI, respectively (P < 0.001). Blockade of ATP-sensitive K+ channels eliminated DynB- and ME-induced protection: 189.6 +/- 5.4 and 139.0 +/- 5.4 for control and ME, respectively (P < 0.001), and 210 +/- 5.9 and 195 +/- 6.1 for 5-HD and ME + 5-HD, respectively (P < 0.001); 136.0 +/- 5.7 and 63.4 +/- 5.4 for control and ME, respectively (P < 0.001), and 144.6 +/- 4.5 and 114.6 +/- 7.7 for HMR-1098 and ME + HMR-1098, respectively (P < 0.01); 189.6 +/- 5.4 and 139.0 +/- 5.4 for control and ME, respectively (P < 0.001), and 210 +/- 5.9 and 195 +/- 6.1 for 5-HD and ME + 5-HD, respectively (P < 0.001); and 136.0 +/- 5.7 and 63.4 +/- 5.4 for control and ME, respectively (P < 0.001), and 144.6 +/- 4.5 and 114.6 +/- 7.7 for HMR-1098 and ME + HMR-1098, respectively (P < 0.01). We conclude that opioid peptide-induced cardioprotection is mediated by delta- and kappa-receptors and involves sarcolemmal and mitochondrial ATP-sensitive K+ channels.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adenosine Triphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Benzamides,
http://linkedlifedata.com/resource/pubmed/chemical/Cardiotonic Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Dynorphins,
http://linkedlifedata.com/resource/pubmed/chemical/Endorphins,
http://linkedlifedata.com/resource/pubmed/chemical/Enkephalin, Methionine,
http://linkedlifedata.com/resource/pubmed/chemical/GNTI compound,
http://linkedlifedata.com/resource/pubmed/chemical/HMR 1098,
http://linkedlifedata.com/resource/pubmed/chemical/Naltrexone,
http://linkedlifedata.com/resource/pubmed/chemical/Narcotic Antagonists,
http://linkedlifedata.com/resource/pubmed/chemical/Potassium Channels,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Opioid, delta,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Opioid, kappa,
http://linkedlifedata.com/resource/pubmed/chemical/naltrindole,
http://linkedlifedata.com/resource/pubmed/chemical/rimorphin
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| pubmed:status |
MEDLINE
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| pubmed:month |
Sep
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| pubmed:issn |
0363-6135
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
285
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
H1032-9
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:12730057-Adenosine Triphosphate,
pubmed-meshheading:12730057-Animals,
pubmed-meshheading:12730057-Benzamides,
pubmed-meshheading:12730057-Cardiotonic Agents,
pubmed-meshheading:12730057-Dose-Response Relationship, Drug,
pubmed-meshheading:12730057-Dynorphins,
pubmed-meshheading:12730057-Endorphins,
pubmed-meshheading:12730057-Enkephalin, Methionine,
pubmed-meshheading:12730057-Ischemic Preconditioning, Myocardial,
pubmed-meshheading:12730057-Male,
pubmed-meshheading:12730057-Mitochondria,
pubmed-meshheading:12730057-Myocardial Ischemia,
pubmed-meshheading:12730057-Myocytes, Cardiac,
pubmed-meshheading:12730057-Naltrexone,
pubmed-meshheading:12730057-Narcotic Antagonists,
pubmed-meshheading:12730057-Potassium Channels,
pubmed-meshheading:12730057-Rabbits,
pubmed-meshheading:12730057-Receptors, Opioid, delta,
pubmed-meshheading:12730057-Receptors, Opioid, kappa,
pubmed-meshheading:12730057-Sarcolemma
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| pubmed:year |
2003
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| pubmed:articleTitle |
Activation of delta- and kappa-opioid receptors by opioid peptides protects cardiomyocytes via KATP channels.
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| pubmed:affiliation |
Research and Anesthesiology Services, Veterans Affairs Medical Center, 3710 SW US Veterans Hospital Road, Portland, OR 97201, USA.
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.
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