Linked Life Data

12727852

Source:http://linkedlifedata.com/resource/pubmed/id/12727852

Statements in which the resource exists.
SubjectPredicateObjectContext
pubmed-article:12727852rdf:typepubmed:Citationlld:pubmed
pubmed-article:12727852lifeskim:mentionsumls-concept:C0086418lld:lifeskim
pubmed-article:12727852lifeskim:mentionsumls-concept:C0600139lld:lifeskim
pubmed-article:12727852lifeskim:mentionsumls-concept:C0596901lld:lifeskim
pubmed-article:12727852lifeskim:mentionsumls-concept:C1622501lld:lifeskim
pubmed-article:12727852lifeskim:mentionsumls-concept:C0205242lld:lifeskim
pubmed-article:12727852lifeskim:mentionsumls-concept:C1518174lld:lifeskim
pubmed-article:12727852lifeskim:mentionsumls-concept:C1171362lld:lifeskim
pubmed-article:12727852lifeskim:mentionsumls-concept:C0025543lld:lifeskim
pubmed-article:12727852lifeskim:mentionsumls-concept:C0017262lld:lifeskim
pubmed-article:12727852lifeskim:mentionsumls-concept:C0171955lld:lifeskim
pubmed-article:12727852lifeskim:mentionsumls-concept:C1420841lld:lifeskim
pubmed-article:12727852lifeskim:mentionsumls-concept:C2698300lld:lifeskim
pubmed-article:12727852lifeskim:mentionsumls-concept:C1515670lld:lifeskim
pubmed-article:12727852lifeskim:mentionsumls-concept:C1524075lld:lifeskim
pubmed-article:12727852lifeskim:mentionsumls-concept:C0205263lld:lifeskim
pubmed-article:12727852lifeskim:mentionsumls-concept:C0337112lld:lifeskim
pubmed-article:12727852pubmed:issue9lld:pubmed
pubmed-article:12727852pubmed:dateCreated2003-5-2lld:pubmed
pubmed-article:12727852pubmed:abstractTextDegradation of the extracellular matrix by proteolytic enzymes is a central aspect of physiological and pathologic tissue-remodeling processes such as trophoblastic implantation, wound healing, and tumor invasion. We have hypothesized that prostate adenocarcinoma cell invasion through the normal basal lamina is attributable in part to metalloproteinase-induced cleavage of laminin-5 (Ln-5) and enhanced motility of the cancer cells. We studied the role of membrane type-1-matrix metalloproteinase (MT1-MMP) expressed on the surface of prostate tumor cells in cleaving Ln-5 and enhancing the migration of prostate tumor cells. We also determined the nature of the MT1-MMP cleavage of human Ln-5 and how this altered Ln-5 changes the migration of prostate carcinoma cells. We found that human MT1-MMP cleaves purified human Ln-5 to an 80-kDa fragment. Mass spectrometry analyses of the 80-kDa cleaved product by trypsin and chymotrypsin gave 14 and 9 different peptide sequences, respectively, that were identical to the expected amino acid sequence of the Ln-5-beta3 chain. The recovered peptides represent 14.4% (trypsin) and 10.3% (chymotrypsin) of Ln-5-beta3 chain by amino acid count. Both trypsin and chymotrypsin digestion of MT1-MMP-cleaved product of Ln-5 did not show any other peptides that were identical to the other chains of Ln-5. Using a linear migration assay we found that the Ln-5 cleaved by MT1-MMP enhanced the migration of DU-145 prostate carcinoma cells by 2-fold compared with uncleaved Ln-5. The use of blocked antisense MT1-MMP oligonucleotides inhibited the migration of DU-145 cells on Ln-5. We also found that the prostate carcinoma cells expressing high levels of MT1-MMP, such as PC3N and PPC, demonstrated enhanced migration on human Ln-5-coated substrate, and this migration was inhibited using blocked antisense MT1-MMP oligonucleotides. In conclusion, this is a novel and important finding where we have shown that beta3-chain is cleaved by MT1-MMP, and this cleavage enhances migration of prostate cancer cells.lld:pubmed
pubmed-article:12727852pubmed:granthttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:12727852pubmed:granthttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:12727852pubmed:granthttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:12727852pubmed:granthttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:12727852pubmed:languageenglld:pubmed
pubmed-article:12727852pubmed:journalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:12727852pubmed:citationSubsetIMlld:pubmed
pubmed-article:12727852pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:12727852pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:12727852pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:12727852pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:12727852pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:12727852pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:12727852pubmed:statusMEDLINElld:pubmed
pubmed-article:12727852pubmed:monthMaylld:pubmed
pubmed-article:12727852pubmed:issn0008-5472lld:pubmed
pubmed-article:12727852pubmed:authorpubmed-author:NagleRaymond...lld:pubmed
pubmed-article:12727852pubmed:authorpubmed-author:CressAnne EAElld:pubmed
pubmed-article:12727852pubmed:authorpubmed-author:BowdenG...lld:pubmed
pubmed-article:12727852pubmed:authorpubmed-author:UdayakumarThi...lld:pubmed
pubmed-article:12727852pubmed:authorpubmed-author:ChenMan...lld:pubmed
pubmed-article:12727852pubmed:authorpubmed-author:BairElisabeth...lld:pubmed
pubmed-article:12727852pubmed:authorpubmed-author:Von...lld:pubmed
pubmed-article:12727852pubmed:issnTypePrintlld:pubmed
pubmed-article:12727852pubmed:day1lld:pubmed
pubmed-article:12727852pubmed:volume63lld:pubmed
pubmed-article:12727852pubmed:ownerNLMlld:pubmed
pubmed-article:12727852pubmed:authorsCompleteYlld:pubmed
pubmed-article:12727852pubmed:pagination2292-9lld:pubmed
pubmed-article:12727852pubmed:dateRevised2009-10-6lld:pubmed
pubmed-article:12727852pubmed:meshHeadingpubmed-meshheading:12727852...lld:pubmed
pubmed-article:12727852pubmed:meshHeadingpubmed-meshheading:12727852...lld:pubmed
pubmed-article:12727852pubmed:meshHeadingpubmed-meshheading:12727852...lld:pubmed
pubmed-article:12727852pubmed:meshHeadingpubmed-meshheading:12727852...lld:pubmed
pubmed-article:12727852pubmed:meshHeadingpubmed-meshheading:12727852...lld:pubmed
pubmed-article:12727852pubmed:meshHeadingpubmed-meshheading:12727852...lld:pubmed
pubmed-article:12727852pubmed:meshHeadingpubmed-meshheading:12727852...lld:pubmed
pubmed-article:12727852pubmed:meshHeadingpubmed-meshheading:12727852...lld:pubmed
pubmed-article:12727852pubmed:meshHeadingpubmed-meshheading:12727852...lld:pubmed
pubmed-article:12727852pubmed:meshHeadingpubmed-meshheading:12727852...lld:pubmed
pubmed-article:12727852pubmed:meshHeadingpubmed-meshheading:12727852...lld:pubmed
pubmed-article:12727852pubmed:meshHeadingpubmed-meshheading:12727852...lld:pubmed
pubmed-article:12727852pubmed:meshHeadingpubmed-meshheading:12727852...lld:pubmed
pubmed-article:12727852pubmed:meshHeadingpubmed-meshheading:12727852...lld:pubmed
pubmed-article:12727852pubmed:year2003lld:pubmed
pubmed-article:12727852pubmed:articleTitleMembrane type-1-matrix metalloproteinase expressed by prostate carcinoma cells cleaves human laminin-5 beta3 chain and induces cell migration.lld:pubmed
pubmed-article:12727852pubmed:affiliationDepartment of Radiation Oncology, Arizona Cancer Center, The University of Arizona, Tucson 85724-5024, USA.lld:pubmed
pubmed-article:12727852pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:12727852pubmed:publicationTypeResearch Support, U.S. Gov't, P.H.S.lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:12727852lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:12727852lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:12727852lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:12727852lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:12727852lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:12727852lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:12727852lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:12727852lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:12727852lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:12727852lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:12727852lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:12727852lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:12727852lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:12727852lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:12727852lld:pubmed