Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2003-4-30
pubmed:abstractText
Isolation and purification of bioproducts from crude extracts can be obtained by affinity methods based on reversible binding of a specific molecule to ligand immobilized in a porous matrix. In the present work, nicrospheres based on chitosan matrix, which incorporated aminophenylboronic acid as a derivative, were prepared and characterized, aimed at developing a beta-amylase adsorption process. Kinetic curves and adsorption isotheriru of the crude extracts as well as the breakthrough curves for a frontal chromatographic separation method of a commercial sample of beta-amylase from soybean are presented. These results were compared to similar data obtained with a comercial microspheres gel based-on agarose.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0273-2289
pubmed:author
pubmed:issnType
Print
pubmed:volume
105 -108
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
829-42
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
Phenylboronate-chitosan resins for adsorption of beta-amylase from soybean extracts.
pubmed:affiliation
Pharmacy and Biochemistry, Dom Bosco Catholic University, UCDB, C.P. 100, CEP 79117-900, Campo Grande, MS, Brazil.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't