Source:http://linkedlifedata.com/resource/pubmed/id/12721421
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:dateCreated |
2003-4-30
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pubmed:abstractText |
Isolation and purification of bioproducts from crude extracts can be obtained by affinity methods based on reversible binding of a specific molecule to ligand immobilized in a porous matrix. In the present work, nicrospheres based on chitosan matrix, which incorporated aminophenylboronic acid as a derivative, were prepared and characterized, aimed at developing a beta-amylase adsorption process. Kinetic curves and adsorption isotheriru of the crude extracts as well as the breakthrough curves for a frontal chromatographic separation method of a commercial sample of beta-amylase from soybean are presented. These results were compared to similar data obtained with a comercial microspheres gel based-on agarose.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Boronic Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Chitin,
http://linkedlifedata.com/resource/pubmed/chemical/Chitosan,
http://linkedlifedata.com/resource/pubmed/chemical/Composite Resins,
http://linkedlifedata.com/resource/pubmed/chemical/benzeneboronic acid,
http://linkedlifedata.com/resource/pubmed/chemical/beta-Amylase
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pubmed:status |
MEDLINE
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pubmed:issn |
0273-2289
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
105 -108
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
829-42
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:12721421-Adsorption,
pubmed-meshheading:12721421-Boronic Acids,
pubmed-meshheading:12721421-Chitin,
pubmed-meshheading:12721421-Chitosan,
pubmed-meshheading:12721421-Composite Resins,
pubmed-meshheading:12721421-Kinetics,
pubmed-meshheading:12721421-Soybeans,
pubmed-meshheading:12721421-beta-Amylase
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pubmed:year |
2003
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pubmed:articleTitle |
Phenylboronate-chitosan resins for adsorption of beta-amylase from soybean extracts.
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pubmed:affiliation |
Pharmacy and Biochemistry, Dom Bosco Catholic University, UCDB, C.P. 100, CEP 79117-900, Campo Grande, MS, Brazil.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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