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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
28
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pubmed:dateCreated |
2003-7-4
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pubmed:abstractText |
Coenzyme Q (Q) is a lipid that functions as an electron carrier in the mitochondrial respiratory chain in eukaryotes. There are eight complementation groups of Q-deficient Saccharomyces cerevisiae mutants, designated coq1-coq8. Here we have isolated the COQ6 gene by functional complementation and, in contrast to a previous report, find it is not an essential gene. coq6 mutants are unable to grow on nonfermentable carbon sources and do not synthesize Q but instead accumulate the Q biosynthetic intermediate 3-hexaprenyl-4-hydroxybenzoic acid. The Coq6 polypeptide is imported into the mitochondria in a membrane potential-dependent manner. Coq6p is a peripheral membrane protein that localizes to the matrix side of the inner mitochondrial membrane. Based on sequence homology to known proteins, we suggest that COQ6 encodes a flavin-dependent monooxygenase required for one or more steps in Q biosynthesis.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
11
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pubmed:volume |
278
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
25308-16
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:12721307-Amino Acid Motifs,
pubmed-meshheading:12721307-Amino Acid Sequence,
pubmed-meshheading:12721307-Carbon,
pubmed-meshheading:12721307-Cell Division,
pubmed-meshheading:12721307-Chromatography, High Pressure Liquid,
pubmed-meshheading:12721307-Cloning, Molecular,
pubmed-meshheading:12721307-Escherichia coli,
pubmed-meshheading:12721307-Flavins,
pubmed-meshheading:12721307-Gene Deletion,
pubmed-meshheading:12721307-Genetic Complementation Test,
pubmed-meshheading:12721307-Genotype,
pubmed-meshheading:12721307-Hydroxybenzoic Acids,
pubmed-meshheading:12721307-Immunoblotting,
pubmed-meshheading:12721307-Membrane Potentials,
pubmed-meshheading:12721307-Mitochondria,
pubmed-meshheading:12721307-Mixed Function Oxygenases,
pubmed-meshheading:12721307-Models, Chemical,
pubmed-meshheading:12721307-Models, Genetic,
pubmed-meshheading:12721307-Molecular Sequence Data,
pubmed-meshheading:12721307-Mutation,
pubmed-meshheading:12721307-Plasmids,
pubmed-meshheading:12721307-Protein Binding,
pubmed-meshheading:12721307-Saccharomyces cerevisiae,
pubmed-meshheading:12721307-Sequence Homology, Amino Acid,
pubmed-meshheading:12721307-Triterpenes,
pubmed-meshheading:12721307-Ubiquinone
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pubmed:year |
2003
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pubmed:articleTitle |
The Saccharomyces cerevisiae COQ6 gene encodes a mitochondrial flavin-dependent monooxygenase required for coenzyme Q biosynthesis.
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pubmed:affiliation |
Department of Chemistry and Biochemistry and the Molecular Biology Institute, University of California, Los Angeles, California 90095, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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