Source:http://linkedlifedata.com/resource/pubmed/id/12718902
Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
5 Pt 1
|
pubmed:dateCreated |
2003-4-29
|
pubmed:abstractText |
In this study the effect of local adenoviral-mediated delivery of inducible nitric oxide synthase on restenosis was evaluated in a porcine coronary stented model. Local gene transfer of recombinant adenoviral vectors that encode human inducible nitric oxide synthase (AdiNOS) was tested. Control vector (AdNull) lacked a recombinant transgene. Endoluminal delivery of 1.0 x 10(11) adenoviral particles was accomplished in 45 s using the Infiltrator catheter (Interventional Technologies, San Diego, CA). Coronary stents were deployed, oversized by a ratio of 1.2:1, in the treated segments immediately after gene transfer. Fourteen animals were sacrificed at day 28 to evaluate the effects of iNOS gene transfer on morphometric indices, and 4 animals were sacrificed at day 4 for detection of human iNOS expression by RT-PCR. iNOS mRNA was detected in six of eight iNOS-transferred arteries, whereas no expression of human iNOS was detected in the nontarget arteries. Morphometric analysis showed that iNOS transfer significantly reduced neointimal formation (3.41 +/- 1.12 mm(2) vs 2.14 +/- 0.68 mm(2), P < 0.05). We concluded that efficient intramural adenovirus-mediated iNOS transfer can be achieved by using Infiltrator catheters. iNOS gene transfer significantly reduces neointimal hyperplasia following stent injury.
|
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
May
|
pubmed:issn |
1525-0016
|
pubmed:author |
pubmed-author:BroughDouglas EDE,
pubmed-author:ForudiFarhadF,
pubmed-author:KesslerPaul DPD,
pubmed-author:KibbeMelinaM,
pubmed-author:KovesdiImreI,
pubmed-author:LincoffA MichaelAM,
pubmed-author:PennMarc SMS,
pubmed-author:TarakjiKhaldounK,
pubmed-author:TopolEric JEJ,
pubmed-author:VEGAYY,
pubmed-author:ZhouXiaorongX,
pubmed-author:ZhouZhongminZ
|
pubmed:issnType |
Print
|
pubmed:volume |
7
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
597-603
|
pubmed:dateRevised |
2011-10-27
|
pubmed:meshHeading |
pubmed-meshheading:12718902-Adenoviridae,
pubmed-meshheading:12718902-Animals,
pubmed-meshheading:12718902-Arterial Occlusive Diseases,
pubmed-meshheading:12718902-Coronary Stenosis,
pubmed-meshheading:12718902-Coronary Vessels,
pubmed-meshheading:12718902-Gene Therapy,
pubmed-meshheading:12718902-Gene Transfer Techniques,
pubmed-meshheading:12718902-Genetic Vectors,
pubmed-meshheading:12718902-Humans,
pubmed-meshheading:12718902-Immunoenzyme Techniques,
pubmed-meshheading:12718902-Nitric Oxide Synthase,
pubmed-meshheading:12718902-Nitric Oxide Synthase Type II,
pubmed-meshheading:12718902-RNA, Messenger,
pubmed-meshheading:12718902-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:12718902-Stents,
pubmed-meshheading:12718902-Swine,
pubmed-meshheading:12718902-Swine, Miniature,
pubmed-meshheading:12718902-Transfection,
pubmed-meshheading:12718902-Tunica Intima
|
pubmed:year |
2003
|
pubmed:articleTitle |
Local adenoviral-mediated inducible nitric oxide synthase gene transfer inhibits neointimal formation in the porcine coronary stented model.
|
pubmed:affiliation |
Experimental Animal Laboratory, Department of Cardiovascular Medicine, The Cleveland Clinic Foundation, Ohio 44195, USA. wangk@ccf.org
|
pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|