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PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5 Pt 1
pubmed:dateCreated
2003-4-29
pubmed:abstractText
In this study the effect of local adenoviral-mediated delivery of inducible nitric oxide synthase on restenosis was evaluated in a porcine coronary stented model. Local gene transfer of recombinant adenoviral vectors that encode human inducible nitric oxide synthase (AdiNOS) was tested. Control vector (AdNull) lacked a recombinant transgene. Endoluminal delivery of 1.0 x 10(11) adenoviral particles was accomplished in 45 s using the Infiltrator catheter (Interventional Technologies, San Diego, CA). Coronary stents were deployed, oversized by a ratio of 1.2:1, in the treated segments immediately after gene transfer. Fourteen animals were sacrificed at day 28 to evaluate the effects of iNOS gene transfer on morphometric indices, and 4 animals were sacrificed at day 4 for detection of human iNOS expression by RT-PCR. iNOS mRNA was detected in six of eight iNOS-transferred arteries, whereas no expression of human iNOS was detected in the nontarget arteries. Morphometric analysis showed that iNOS transfer significantly reduced neointimal formation (3.41 +/- 1.12 mm(2) vs 2.14 +/- 0.68 mm(2), P < 0.05). We concluded that efficient intramural adenovirus-mediated iNOS transfer can be achieved by using Infiltrator catheters. iNOS gene transfer significantly reduces neointimal hyperplasia following stent injury.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1525-0016
pubmed:author
pubmed:issnType
Print
pubmed:volume
7
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
597-603
pubmed:dateRevised
2011-10-27
pubmed:meshHeading
pubmed-meshheading:12718902-Adenoviridae, pubmed-meshheading:12718902-Animals, pubmed-meshheading:12718902-Arterial Occlusive Diseases, pubmed-meshheading:12718902-Coronary Stenosis, pubmed-meshheading:12718902-Coronary Vessels, pubmed-meshheading:12718902-Gene Therapy, pubmed-meshheading:12718902-Gene Transfer Techniques, pubmed-meshheading:12718902-Genetic Vectors, pubmed-meshheading:12718902-Humans, pubmed-meshheading:12718902-Immunoenzyme Techniques, pubmed-meshheading:12718902-Nitric Oxide Synthase, pubmed-meshheading:12718902-Nitric Oxide Synthase Type II, pubmed-meshheading:12718902-RNA, Messenger, pubmed-meshheading:12718902-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:12718902-Stents, pubmed-meshheading:12718902-Swine, pubmed-meshheading:12718902-Swine, Miniature, pubmed-meshheading:12718902-Transfection, pubmed-meshheading:12718902-Tunica Intima
pubmed:year
2003
pubmed:articleTitle
Local adenoviral-mediated inducible nitric oxide synthase gene transfer inhibits neointimal formation in the porcine coronary stented model.
pubmed:affiliation
Experimental Animal Laboratory, Department of Cardiovascular Medicine, The Cleveland Clinic Foundation, Ohio 44195, USA. wangk@ccf.org
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't