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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
2003-4-28
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pubmed:abstractText |
Embryonic stem (ES) cells provide a unique source for tissue regeneration. We examined whether mouse ES cells can efficiently differentiate into transplantable hepatocytes. ES cells were implanted into mouse livers 24 hours after carbon tetrachloride intoxication; ES-derived cells with several hepatocyte-cell-markers were generated. They were able to grow in vitro and showed morphology consistent with typical mature hepatocytes and expressed hepatocyte-specific genes. After transplantation into the carbon tetrachloride-injured mouse liver, ES-derived green fluorescent protein-positive cells were incorporated into liver tissue and rescued mice from hepatic injury. No teratoma formation was observed in the transplant recipients. In conclusion, ES cells can provide a valuable tool for studying the molecular basis for differentiation of hepatocytes and form the basis for cell therapies.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0270-9139
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
37
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
983-93
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:12717379-Albumins,
pubmed-meshheading:12717379-Animals,
pubmed-meshheading:12717379-Carbon Tetrachloride,
pubmed-meshheading:12717379-Cell Differentiation,
pubmed-meshheading:12717379-Cells, Cultured,
pubmed-meshheading:12717379-Drug-Induced Liver Injury,
pubmed-meshheading:12717379-Female,
pubmed-meshheading:12717379-Green Fluorescent Proteins,
pubmed-meshheading:12717379-Hepatocytes,
pubmed-meshheading:12717379-Immunohistochemistry,
pubmed-meshheading:12717379-Indicators and Reagents,
pubmed-meshheading:12717379-Liver Regeneration,
pubmed-meshheading:12717379-Luminescent Proteins,
pubmed-meshheading:12717379-Male,
pubmed-meshheading:12717379-Mice,
pubmed-meshheading:12717379-Mice, Inbred Strains,
pubmed-meshheading:12717379-Stem Cell Transplantation,
pubmed-meshheading:12717379-Stem Cells
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pubmed:year |
2003
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pubmed:articleTitle |
Differentiation of embryonic stem cells into hepatocytes: biological functions and therapeutic application.
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pubmed:affiliation |
National Cancer Center Research Institute, Tokyo, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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