Linked Life Data

12716929

Source:http://linkedlifedata.com/resource/pubmed/id/12716929

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
2003-4-28
pubmed:abstractText
Demyelinating diseases can be associated with painful sensory phenomena such as tactile allodynia and hyperalgesia. To study the mechanisms underlying demyelination-induced pain, we have characterized a novel model of demyelination of the sciatic or saphenous nerve. Topical lysolecithin application causes focal demyelination of afferent nerve A-fibers without axonal loss, as assessed either by electron and light microscopy or by immunohistochemical analysis of dorsal root ganglia (DRG) for a neuronal injury marker, activating transcription factor 3. Focal demyelination is accompanied by spontaneous action potentials in afferents and increased expression of neuropeptide Y and Na(v)1.3 sodium channels specifically in DRG neurons that coexpress a specific marker of myelinated afferents. In contrast, expression of tetrodotoxin-resistant, Na(v)1.8 sodium channels is specifically decreased in the same subgroup of DRG cells. Central sensitization of somatosensory processing is also induced, with increased behavioral reflex responsiveness to thermal and mechanical stimuli. These changes are reversed by intrathecal administration of an NMDA receptor antagonist or cannabinoid (CB) receptor agonist, but not by a mu-opioid receptor agonist. Recovery of behavioral reflexes occurred approximately 3 weeks after lysolecithin treatment. This is the first time that demyelination of afferent A-fibers has been shown to specifically induce neuropathic pain and indicates that axonal damage is not a prerequisite for development of the pain state. The profile of phenotypic changes in DRG is distinct from other pain models and displays a sensitivity to NMDA and CB receptor agents that may be exploitable therapeutically.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1529-2401
pubmed:author
pubmed:issnType
Electronic
pubmed:day
15
pubmed:volume
23
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3221-33
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:12716929-Action Potentials, pubmed-meshheading:12716929-Animals, pubmed-meshheading:12716929-Behavior, Animal, pubmed-meshheading:12716929-Cannabinoids, pubmed-meshheading:12716929-Demyelinating Diseases, pubmed-meshheading:12716929-Disease Models, Animal, pubmed-meshheading:12716929-Drug Administration Routes, pubmed-meshheading:12716929-Excitatory Amino Acid Antagonists, pubmed-meshheading:12716929-Ganglia, Spinal, pubmed-meshheading:12716929-Immunohistochemistry, pubmed-meshheading:12716929-Lysophosphatidylcholines, pubmed-meshheading:12716929-Mice, pubmed-meshheading:12716929-Mice, Inbred C57BL, pubmed-meshheading:12716929-Mononeuropathies, pubmed-meshheading:12716929-Nerve Fibers, Myelinated, pubmed-meshheading:12716929-Neurons, Afferent, pubmed-meshheading:12716929-Neuropeptide Y, pubmed-meshheading:12716929-Pain, pubmed-meshheading:12716929-Peripheral Nerves, pubmed-meshheading:12716929-Receptors, N-Methyl-D-Aspartate, pubmed-meshheading:12716929-Receptors, Opioid, mu, pubmed-meshheading:12716929-Reflex, pubmed-meshheading:12716929-Sciatic Nerve, pubmed-meshheading:12716929-Sodium Channels
pubmed:year
2003
pubmed:articleTitle
Focal lysolecithin-induced demyelination of peripheral afferents results in neuropathic pain behavior that is attenuated by cannabinoids.
pubmed:affiliation
Centre for Neuroscience Research, Division of Preclinical Veterinary Sciences, The Royal (Dick) School of Veterinary Studies, The University of Edinburgh, Summerhall, Edinburgh EH9 1QH, United Kingdom.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't