Source:http://linkedlifedata.com/resource/pubmed/id/12714266
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
2003-4-25
|
| pubmed:abstractText |
In the present investigation we analysed Interleukin 6 (IL-6) in vitro production by Epstein-Barr virus (EBV)-immortalized B lymphocytes established from 43 subjects, 15 young people and 28 elderly people, including 18 centenarians, after 3, 6, 9, 24, 48 and 72 h of culture. The subjects were genotypized for the C to G transition at nucleotide -174 of IL-6 gene promoter (-174 C/G) and were classified as C allele carriers (C+) and non-carriers (C-). We found that: (i) the interindividual difference in in vitro IL-6 production was wider in elderly individuals in respect to young individuals, leading to different coefficient of variation in the two groups; (ii) the -174 C/G polymorphism had an age-related effect on IL-6 in vitro production. Only among C- people, cells from elderly subjects produced significant higher level of IL-6 than cells from young subjects. These data are consistent with our previous results regarding the IL-6 serum levels in a large group of people of different age, including centenarians. Thus, the EBV-immortalized B lymphocytes can be considered a useful in vitro model for studying the genetic control of IL-6 production and its changes with age.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Apr
|
| pubmed:issn |
0047-6374
|
| pubmed:author |
pubmed-author:BonafèMassimilianoM,
pubmed-author:CardelliMaurizioM,
pubmed-author:CarrieriGiuseppinaG,
pubmed-author:CavalloneLucaL,
pubmed-author:CenturelliMatteoM,
pubmed-author:FalsettiLorenzoL,
pubmed-author:FranceschiClaudioC,
pubmed-author:GiampieriClaudiaC,
pubmed-author:GiovagnettiSimonaS,
pubmed-author:LisaRosamariaR,
pubmed-author:MarchegianiFrancescaF,
pubmed-author:MoresiRaffaellaR,
pubmed-author:MugianesiElenaE,
pubmed-author:OlivieriFabiolaF,
pubmed-author:SalvioliStefanoS,
pubmed-author:SpazzafumoLianaL,
pubmed-author:StecconiRosaliaR,
pubmed-author:TeseiSilviaS,
pubmed-author:ViticchiClaudioC
|
| pubmed:issnType |
Print
|
| pubmed:volume |
124
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
549-53
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:12714266-Adult,
pubmed-meshheading:12714266-Aged,
pubmed-meshheading:12714266-Aged, 80 and over,
pubmed-meshheading:12714266-Aging,
pubmed-meshheading:12714266-B-Lymphocytes,
pubmed-meshheading:12714266-Cell Line, Transformed,
pubmed-meshheading:12714266-Female,
pubmed-meshheading:12714266-Genotype,
pubmed-meshheading:12714266-Herpesvirus 4, Human,
pubmed-meshheading:12714266-Humans,
pubmed-meshheading:12714266-Interleukin-6,
pubmed-meshheading:12714266-Male,
pubmed-meshheading:12714266-Middle Aged,
pubmed-meshheading:12714266-Polymorphism, Genetic
|
| pubmed:year |
2003
|
| pubmed:articleTitle |
In vitro IL-6 production by EBV-immortalized B lymphocytes from young and elderly people genotyped for -174 C/G polymorphism in IL-6 gene: a model to study the genetic basis of inflamm-aging.
|
| pubmed:affiliation |
Italian National Research Centers on Aging (I.N.R.C.A.), Via S. Margherita, n. 5, 60121, Ancona, Italy.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
In Vitro,
Research Support, Non-U.S. Gov't
|