Source:http://linkedlifedata.com/resource/pubmed/id/12713918
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
7
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pubmed:dateCreated |
2003-4-25
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pubmed:abstractText |
Arrays of nanoscopic gold tubes were prepared by electroless deposition of the metal within the pores of polycarbonate particle track-etched membranes (PTM). Glucose oxidase (Gox) was immobilised onto preformed self-assembled monolayers (SAMs) (mercaptoethylamine or mercaptopropionic acid) on electroless gold via cross-linking with glutaraldehyde or covalent attachment by carbodiimide coupling. The effectiveness of the different steps in the Gox immobilisation procedure was assessed by contact angle measurements, cyclic voltammetry and X-ray photoelectron spectroscopy. The enzyme loading was estimated by radioactivity measurements. The sensitivity to beta-glucose of these different biosensors has been evaluated. Glucose responses as large as 400 nA mM(-1) cm(-2) have been obtained. To our knowledge, this sensitivity value is amongst the highest values reported in the literature for comparable biosensor systems.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cross-Linking Reagents,
http://linkedlifedata.com/resource/pubmed/chemical/Enzymes, Immobilized,
http://linkedlifedata.com/resource/pubmed/chemical/Glucose,
http://linkedlifedata.com/resource/pubmed/chemical/Glucose Oxidase,
http://linkedlifedata.com/resource/pubmed/chemical/Gold
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0956-5663
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
18
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
943-51
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pubmed:dateRevised |
2009-7-14
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pubmed:meshHeading |
pubmed-meshheading:12713918-Biosensing Techniques,
pubmed-meshheading:12713918-Cross-Linking Reagents,
pubmed-meshheading:12713918-Electrochemistry,
pubmed-meshheading:12713918-Enzymes, Immobilized,
pubmed-meshheading:12713918-Glucose,
pubmed-meshheading:12713918-Glucose Oxidase,
pubmed-meshheading:12713918-Gold,
pubmed-meshheading:12713918-Microscopy, Electron, Scanning,
pubmed-meshheading:12713918-Nanotechnology,
pubmed-meshheading:12713918-Nanotubes
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pubmed:year |
2003
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pubmed:articleTitle |
Immobilisation of glucose oxidase within metallic nanotubes arrays for application to enzyme biosensors.
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pubmed:affiliation |
Unité de Chimie et de Physique des Hauts Polymères, Université Catholique de Louvain, Place Croix du Sud, 1, B-1348, Louvain-la-Neuve, Belgium.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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