Linked Life Data

12713041

Source:http://linkedlifedata.com/resource/pubmed/id/12713041

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
2003-4-25
pubmed:abstractText
The present study describes the effect of urea on the properties of hydroxyethyl cellulose (HEC) polymer solutions used for the separation of nucleic acids. Solution properties were investigated by viscosity measurements and supplemented by Raman spectroscopy of the solution components. By using viscosimetry, it was possible to identify that borate, urea, and HEC participate in an interaction causing an increase in the viscosity of dilute solutions. In addition, short polymer chains exhibited a 4-fold decrease in the entanglement threshold in the presence of 4 M urea and 0.356 M total borate concentration. These interactions were found to be specific to HEC. Raman spectroscopy monitored serial addition of 8 M urea to HEC sieving solutions combined with factor analysis indicated formation of minor urea species. Lack of change in the Raman spectrum and relative amount of borate suggested that there is no direct interaction between borate and urea. These effects on HEC sieving solution properties lead to the use of low HEC concentrations that are beneficial for the separation of nucleic acids under denaturing conditions.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0003-2700
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
75
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1837-43
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
Effect of urea on the polymer buffer solutions used for the electrophoretic separations of nucleic acids.
pubmed:affiliation
Department of Chemistry, University of Michigan, Ann Arbor, Michigan 48109-1055, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.