Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2-3
pubmed:dateCreated
2003-4-24
pubmed:abstractText
Alteration of androgen receptor function due to hormonally active compounds in the environment, may be responsible for impaired reproductive function in aquatic wildlife. Based on human prostate carcinoma 22RV1 cells, a cell culture expression system was established to test effects of putative androgenic/antiandrogenic compounds on endogenous gene expression. 22RV1 cells were shown to express human androgen receptor, but not human progestin (hPR) or human oestrogen receptor (hER) alpha and beta. Six androgen-regulated genes (ARGs) were chosen to determine androgenic/antiandrogenic action using highly sensitive real-time RT-PCR. Results showed that gene expression is altered in a time-dependent manner. After stimulation of cells by DHT (10nM), synthetic androgen R1881 (1 nM), or organic pesticides (difenoconazole, fentinacetate, tetramethrin) TMPRSS2 mRNA expression was down-regulated by the factor 0.6 after 24h of DHT treatment. Similar results were obtained when cells were assayed for mRNA expression of PSA after fentinacetate and R1881 stimulation. In contrast, TMPRSS2 expression was up-regulated by the factor 0.9 when cells were stimulated by tetramethrin. Final goal of the work is a sensitive determination of differential gene expression by different compounds under study, achievement of substance-specific expression patterns and function related analysis of potential androgens/antiandrogens.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Androgen Antagonists, http://linkedlifedata.com/resource/pubmed/chemical/Androgens, http://linkedlifedata.com/resource/pubmed/chemical/Dioxolanes, http://linkedlifedata.com/resource/pubmed/chemical/Estrogen Receptor alpha, http://linkedlifedata.com/resource/pubmed/chemical/Estrogen Receptor beta, http://linkedlifedata.com/resource/pubmed/chemical/Fungicides, Industrial, http://linkedlifedata.com/resource/pubmed/chemical/Insecticides, http://linkedlifedata.com/resource/pubmed/chemical/Organotin Compounds, http://linkedlifedata.com/resource/pubmed/chemical/Pesticides, http://linkedlifedata.com/resource/pubmed/chemical/Progestins, http://linkedlifedata.com/resource/pubmed/chemical/Pyrethrins, http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger, http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Androgen, http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Estrogen, http://linkedlifedata.com/resource/pubmed/chemical/Teratogens, http://linkedlifedata.com/resource/pubmed/chemical/Triazoles, http://linkedlifedata.com/resource/pubmed/chemical/difenoconazole, http://linkedlifedata.com/resource/pubmed/chemical/phentin acetate, http://linkedlifedata.com/resource/pubmed/chemical/tetramethrin
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0960-0760
pubmed:author
pubmed:issnType
Print
pubmed:volume
84
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
231-8
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed-meshheading:12711008-Androgen Antagonists, pubmed-meshheading:12711008-Androgens, pubmed-meshheading:12711008-Cell Division, pubmed-meshheading:12711008-Dioxolanes, pubmed-meshheading:12711008-Down-Regulation, pubmed-meshheading:12711008-Environmental Exposure, pubmed-meshheading:12711008-Estrogen Receptor alpha, pubmed-meshheading:12711008-Estrogen Receptor beta, pubmed-meshheading:12711008-Fungicides, Industrial, pubmed-meshheading:12711008-Gene Expression, pubmed-meshheading:12711008-Humans, pubmed-meshheading:12711008-Insecticides, pubmed-meshheading:12711008-Male, pubmed-meshheading:12711008-Organotin Compounds, pubmed-meshheading:12711008-Pesticides, pubmed-meshheading:12711008-Progestins, pubmed-meshheading:12711008-Prostatic Neoplasms, pubmed-meshheading:12711008-Pyrethrins, pubmed-meshheading:12711008-RNA, Messenger, pubmed-meshheading:12711008-Receptors, Androgen, pubmed-meshheading:12711008-Receptors, Estrogen, pubmed-meshheading:12711008-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:12711008-Teratogens, pubmed-meshheading:12711008-Time Factors, pubmed-meshheading:12711008-Transcription, Genetic, pubmed-meshheading:12711008-Triazoles, pubmed-meshheading:12711008-Tumor Cells, Cultured, pubmed-meshheading:12711008-Up-Regulation
pubmed:year
2003
pubmed:articleTitle
Characterisation of gene expression patterns in 22RV1 cells for determination of environmental androgenic/antiandrogenic compounds.
pubmed:affiliation
Institute of Physiology, Center of Milk and Food Research, Technical University Munich, Weihenstephaner Berg 3, 85354 Freising, Germany. anita.hartel@wzw.tum.de
pubmed:publicationType
Journal Article