Linked Life Data

1270979

Source:http://linkedlifedata.com/resource/pubmed/id/1270979

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1976-8-3
pubmed:abstractText
Young chicken embryos were incubated on media containing formamide at concentrations of 0-1, 0-25, 0-31, 0-37, 0-43 and 0-5 M. In the neuroepithelium of these embryos we found that (1) the 0-1 M concentration had no detectable effect, (2) the 0-25 M concentration only affected mitosis which was blocked in metaphase so that mitotic figures accumulated near the neurocoele, (3) 0-31 M formamide totally inhibited interkinetic nuclear migration and affected only slightly the cell asymmetry, (4) the 0-37 M concentration considerably reduced the amount of cytoplasmic microtubules and that the cells became round, (5) at 0-43 M formamide, all microtubules had disappeared and all cells were spherical, (6) 0-5 M formamide all cells were spherical, detached from one another and the epithelium had lost its usual characteristics. Our results on exposure of the cells to low temperature (2 degrees C) suggest that formamide directly affects microtubules. All the effects observed at concentrations up to 0-43 M formamide are reversible.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0022-0752
pubmed:author
pubmed:issnType
Print
pubmed:volume
35
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
197-212
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1976
pubmed:articleTitle
Effects of formamide on neuroepithelial cells and on interkinetic nuclear migration in the chick embryo.
pubmed:publicationType
Journal Article, In Vitro