Linked Life Data

12707990

Source:http://linkedlifedata.com/resource/pubmed/id/12707990

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2003-4-23
pubmed:abstractText
To evaluate the reliability of our previously reported antimicrobial susceptibility test by ATP method, we have compared our ATP method to the reference test methods such as Mycobacteria Growth Indicator Tube(MGIT) method, minimum inhibitory concentration(MIC) method, NCCLS M24-T agar proportion method(M24-T method), and Vite spectrum method. The concentrations of drugs used for the assessment were isoniazid(INH) 0.1 microgram/ml, rifampicin(RFP) 2.0 micrograms/ml, ethambutol(EB) 2.5 micrograms/ml, streptomycin (SM) 2.0 micrograms/ml, and kanamycin (KM) 5.0 micrograms/ml. When six M. tuberculosis ATCC strains were subjected to 6 independent experiments by using ATP method, highly reproducible results were obtained on the fifth day of the incubation. We examined correlation among ATP method and reference test methods in drug susceptibility testing for 65 clinical isolates of M. tuberculosis. The correlation between ATP method and MGIT-, MIC-, M24-T method were more than 95% for all drugs. When ATP method and Vite spectrum method was compared, the correlation was 87.7% for INH, 98.5% for RFP, 90.8% for EB, 92.3% for SM, 96.9% for KM. The culture period for determining susceptibility between ATP method and MGIT method was compared by using ATCC reference strains and clinical isolates. Six M. tuberculosis ATCC strains were subjected to 6 independent experiments. By the MGIT method, 8 days were required to obtain the results, whereas 3 days were enough by the ATP method. For 65 clinical isolates, the MGIT method required 9 days for determining susceptibility of all isolates. The ATP method required only 5 days for the same strains. These data demonstrate that the improved ATP method that we reported, is simple, rapid, highly reproducible and nonradiometric, and could be used for the assessment of drug susceptibility for M. tuberculosis with high reliability.
pubmed:language
jpn
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0047-1860
pubmed:author
pubmed:issnType
Print
pubmed:volume
51
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
194-200
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
[Reliability of bioluminescent assay (ATP method) for testing antimicrobial susceptibility of Mycobacterium tuberculosis].
pubmed:affiliation
Department of Bacteriology, National Institute of Infectious Diseases, Shinjuku-ku, Tokyo 162-8640.
pubmed:publicationType
Journal Article, Comparative Study, English Abstract, Research Support, Non-U.S. Gov't, Evaluation Studies