Linked Life Data

12707335

Source:http://linkedlifedata.com/resource/pubmed/id/12707335

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
2003-4-22
pubmed:abstractText
We have previously reported that FcgammaR-mediated function in myeloid cells is a tightly regulated event that is influenced by the cytokines present in the milieu. TGF-beta1 is an immunosuppressive cytokine with pleiotropic effects on immune responses; however, the molecular mechanism by which TGF-beta suppresses immune responses is poorly understood. In this study, we have analyzed the effect of TGF-beta on FcgammaR-mediated activation of myeloid cells. We report that TGF-beta1-treated THP-1 human myeloid cells displayed reduced ability to phagocytose IgG-coated particles. Because FcgammaR expression is modulated by cytokines, we analyzed expression levels of FcgammaRI, FcgammaRIIa, FcgammaRIIb, and FcgammaRIIIa in cells cultured with or without TGF-beta1 and found while total protein levels of the FcgammaR were not reduced, surface expression of FcgammaRI and FcgammaRIII was lower in cells cultured with TGF-beta1. Concomitantly, there was a dose-dependent reduction in the expression of the FcgammaR-associated gamma-subunit. This suppressive effect of TGF-beta was likewise observed in bone marrow-derived murine myeloid cells and human monocytes. Importantly, TGF-beta1 also significantly reduced the production of monocyte chemoattractant protein-1 induced by immobilized IgG, which would further reduce monocyte recruitment to the site of inflammation. In contrast, human alveolar macrophages were refractory to this effect, expressing low levels of TGF-beta type II receptors compared with peripheral blood monocytes from the same donor. These data provide insight into the regulation of immune responses by TGF-beta1 and demonstrate the selectivity of these effects.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Chemokine CCL2, http://linkedlifedata.com/resource/pubmed/chemical/IL2RG protein, human, http://linkedlifedata.com/resource/pubmed/chemical/Il2rg protein, mouse, http://linkedlifedata.com/resource/pubmed/chemical/Interleukin Receptor Common gamma..., http://linkedlifedata.com/resource/pubmed/chemical/Protein Subunits, http://linkedlifedata.com/resource/pubmed/chemical/Receptors, IgG, http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Interleukin-7, http://linkedlifedata.com/resource/pubmed/chemical/TGFB1 protein, human, http://linkedlifedata.com/resource/pubmed/chemical/Tgfb1 protein, mouse, http://linkedlifedata.com/resource/pubmed/chemical/Transforming Growth Factor beta, http://linkedlifedata.com/resource/pubmed/chemical/Transforming Growth Factor beta1
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
170
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4572-7
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:12707335-Animals, pubmed-meshheading:12707335-Cell Line, Transformed, pubmed-meshheading:12707335-Chemokine CCL2, pubmed-meshheading:12707335-Down-Regulation, pubmed-meshheading:12707335-Humans, pubmed-meshheading:12707335-Interleukin Receptor Common gamma Subunit, pubmed-meshheading:12707335-Macrophages, Alveolar, pubmed-meshheading:12707335-Mice, pubmed-meshheading:12707335-Monocytes, pubmed-meshheading:12707335-Myeloid Cells, pubmed-meshheading:12707335-Phagocytosis, pubmed-meshheading:12707335-Protein Subunits, pubmed-meshheading:12707335-Receptors, IgG, pubmed-meshheading:12707335-Receptors, Interleukin-7, pubmed-meshheading:12707335-Signal Transduction, pubmed-meshheading:12707335-Transforming Growth Factor beta, pubmed-meshheading:12707335-Transforming Growth Factor beta1, pubmed-meshheading:12707335-Tumor Cells, Cultured
pubmed:year
2003
pubmed:articleTitle
TGF-beta 1 suppresses [correction of supresses] myeloid Fc gamma receptor function by regulating the expression and function of the common gamma-subunit.
pubmed:affiliation
Department of Internal Medicine and Dorothy M. Davis Heart and Lung Research Institute, and The James Cancer Hospital and Comprehensive Cancer Center, Ohio State University, Columbus, OH 43210, USA. tridandapani.2@osu.edu
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't