Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2003-4-22
pubmed:databankReference
pubmed:abstractText
Human CD99 (MIC2) is a 32 kDa cell surface protein and its encoding gene is localized to the pseudoautosomal regions of both Xp and Yp chromosomes. Although sequences of several genes such as human PBDX and MIC2R are known to be related to that of CD99, the murine counterpart of CD99 has not been reported. Here we have identified a novel CD99 mouse paralog, named as CD99L2 (CD99 antigen-like 2), and its human, rat and zebrafish genes. Unlike the rapidly evolved CD99 gene, these CD99L2 genes were highly conserved among those species. However, the genomic organization of human and mouse CD99L2 genes showed a difference in their exon numbers possibly due to exon duplication during evolution. In addition, comparative analysis of the cDNA sequences identified the presence of variants in the region around the exons 3 and 4 even within a species due to a differential splicing event, resulting in species-specific patterns in their transcripts. As determined by in situ hybridization analysis, the CD99L2 gene appeared to be expressed particularly high in neuronal cells despite its ubiquitous distribution. The highly expression on neuronal cells without any variations between species reflects a dominant role of this molecule during neural development. Amino acid sequence alignment revealed five putative functional regions highly conserved between CD99L2 and CD99, indicating a close relationship between the two genes. Moreover, human and mouse CD99L2 were located on their X chromosomes, respectively, whereas the zebrafish mic2l1 gene was in the LG7 chromosome. These observations support the inference that the evolutionary conserved gene, CD99L2, originated from a common ancestor gene of CD99, and its high conservation among species implies at least some essential function.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0378-1119
pubmed:author
pubmed:issnType
Print
pubmed:day
27
pubmed:volume
307
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
63-76
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:12706889-Alternative Splicing, pubmed-meshheading:12706889-Amino Acid Sequence, pubmed-meshheading:12706889-Animals, pubmed-meshheading:12706889-Antigens, CD, pubmed-meshheading:12706889-Base Sequence, pubmed-meshheading:12706889-Cell Line, pubmed-meshheading:12706889-Chromosome Mapping, pubmed-meshheading:12706889-Cloning, Molecular, pubmed-meshheading:12706889-Conserved Sequence, pubmed-meshheading:12706889-DNA, pubmed-meshheading:12706889-DNA, Complementary, pubmed-meshheading:12706889-Evolution, Molecular, pubmed-meshheading:12706889-Exons, pubmed-meshheading:12706889-Female, pubmed-meshheading:12706889-Gene Expression Profiling, pubmed-meshheading:12706889-Genes, pubmed-meshheading:12706889-Humans, pubmed-meshheading:12706889-Introns, pubmed-meshheading:12706889-Male, pubmed-meshheading:12706889-Mice, pubmed-meshheading:12706889-Molecular Sequence Data, pubmed-meshheading:12706889-Phylogeny, pubmed-meshheading:12706889-Rats, pubmed-meshheading:12706889-Sequence Alignment, pubmed-meshheading:12706889-Sequence Analysis, DNA, pubmed-meshheading:12706889-Sequence Homology, Amino Acid, pubmed-meshheading:12706889-Synteny, pubmed-meshheading:12706889-Transcription, Genetic, pubmed-meshheading:12706889-X Chromosome, pubmed-meshheading:12706889-Zebrafish
pubmed:year
2003
pubmed:articleTitle
Cloning, genomic organization, alternative transcripts and expression analysis of CD99L2, a novel paralog of human CD99, and identification of evolutionary conserved motifs.
pubmed:affiliation
Department of Pathology, Seoul National University College of Medicine, 28 Yongon-dong, Chongno-gu, South Korea.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't