Linked Life Data

12703978

Source:http://linkedlifedata.com/resource/pubmed/id/12703978

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
2003-4-21
pubmed:abstractText
A stem cell population isolated from murine skeletal muscle has recently been shown to differentiate into hematopoietic cells after transplantation in vivo. In the present study, we tested the hypothesis that this cell population would also, under appropriate culture conditions, differentiate into skeletal muscle cells in vitro. Lower-extremity skeletal muscle tissue isolated from 3- to 4-wk-old mice was dissected free from bone and vessels, enzymatically digested, and flow cytometrically sorted to yield CD45(-)Sca-1(+)c-Kit(-) (S+) cells. These cells were further sorted into CD34(+) and CD34(-) fractions and examined for skeletal, cardiac, and hematopoietic lineage-specific messenger RNA (mRNA) transcripts immediately after isolation and after a 10- to 14-d culture period. Freshly isolated S(+)CD34(+) cells lacked expression of skeletal-, cardiac-, or hematopoietic-specific mRNA transcripts, whereas S(+)CD34(-) cells expressed c-met, a marker for skeletal muscle satellite cells. During 10-14 d in culture, both S(+)CD34(+) and S(+)CD34(-) cell populations underwent a period of attachment followed by elongation and, ultimately, fusion to create large multinucleated contractile myotubes expressing skeletal muscle lineage mRNA transcripts but not hematopoietic or cardiac lineage transcripts. We conclude that murine skeletal muscle possesses two populations of progenitor cells that can be directly isolated. One population expressing the phenotype S(+)CD34(-) may contain satellite cells, whereas the S(+)CD34(+) population is devoid of satellite cell markers. Both populations possess the ability to differentiate into skeletal muscle cells in vitro.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
1071-2690
pubmed:author
pubmed:issnType
Print
pubmed:volume
38
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
512-7
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:12703978-Adipose Tissue, pubmed-meshheading:12703978-Animals, pubmed-meshheading:12703978-Antigens, Ly, pubmed-meshheading:12703978-Base Sequence, pubmed-meshheading:12703978-Biological Markers, pubmed-meshheading:12703978-Cell Culture Techniques, pubmed-meshheading:12703978-Cell Differentiation, pubmed-meshheading:12703978-DNA Primers, pubmed-meshheading:12703978-Flow Cytometry, pubmed-meshheading:12703978-Lymph Nodes, pubmed-meshheading:12703978-Membrane Proteins, pubmed-meshheading:12703978-Mice, pubmed-meshheading:12703978-Mice, Mutant Strains, pubmed-meshheading:12703978-Muscle, Skeletal, pubmed-meshheading:12703978-Muscle Fibers, Skeletal, pubmed-meshheading:12703978-Organ Culture Techniques, pubmed-meshheading:12703978-Proteins, pubmed-meshheading:12703978-Proto-Oncogene Proteins c-kit, pubmed-meshheading:12703978-RNA, Messenger, pubmed-meshheading:12703978-Skin, pubmed-meshheading:12703978-Stem Cells, pubmed-meshheading:12703978-Transcription, Genetic
pubmed:year
2002
pubmed:articleTitle
Muscle-derived CD45-SCA-1+c-kit- progenitor cells give rise to skeletal muscle myotubes in vitro.
pubmed:affiliation
Department of Pediatrics, Herman B. Wells Center for Pediatric Research, Indiana University School of Medicine, Indianapolis, Indiana 46202, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.