Linked Life Data

12702485

Source:http://linkedlifedata.com/resource/pubmed/id/12702485

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2003-6-9
pubmed:abstractText
An angiotensin (ANG) receptor homologous to the type 1 receptor (AT1) has been cloned in chickens (cAT1). We investigated whether cAT1 expression in various tissues shows maturation/age-dependent changes. cAT1 mRNA levels detected in renal glomeruli [in situ hybridization (ISH)] and kidney extract (RT-PCR) are significantly (P < 0.01) higher in 19-day embryos (EB) than in chicks (CH, 2-3 wk) and pullets/cockerels (PL/CK, 14-16 wk). The levels in adrenal glands (concentrated in subcapsular regions) are high in EB and further increased in CH and PL/CK. cAT1 mRNA is also detectable in smooth muscle (SM)/adventitia of EB and CH aorta and in the adventitia, but not SM, from PL/CK aortas. The endothelia from small arteries and arterioles, but not from aorta, express cAT1 mRNA (ISH). In all age groups, ANG II induces profound endothelium-dependent relaxation of abdominal aorta, partly (37-47%) inhibitable (P < 0.01) by Nomega-nitro-l-arginine methyl ester (l-NAME, 10(-4) M), suggesting the presence of ANG receptor in endothelium. l-NAME-resistant ANG II relaxation, examined in a limited number of EB or CH aortas, was reduced by 125 mM K+ or apamin plus charybdotoxin. The results suggest that 1) cAT1 is present in kidney, adrenal gland, and vascular endothelium (heterogeneity exists among arteries) of EB, CH, and PL/CK, and in aortic SM/adventitia of EB/CH but only in adventitia of PL/CK; 2) levels of cAT1 gene expression change during maturation in a tissue-specific manner; and 3) ANG II-induced relaxation may be partly attributable to nitric oxide and potassium channel activation.
pubmed:grant
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0363-6119
pubmed:author
pubmed:issnType
Print
pubmed:volume
285
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
R231-42
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:12702485-Adrenal Glands, pubmed-meshheading:12702485-Angiotensin II, pubmed-meshheading:12702485-Animals, pubmed-meshheading:12702485-Aorta, Abdominal, pubmed-meshheading:12702485-Chick Embryo, pubmed-meshheading:12702485-Chickens, pubmed-meshheading:12702485-Gene Expression Regulation, Developmental, pubmed-meshheading:12702485-In Situ Hybridization, pubmed-meshheading:12702485-Kidney Glomerulus, pubmed-meshheading:12702485-RNA, Messenger, pubmed-meshheading:12702485-Receptor, Angiotensin, Type 1, pubmed-meshheading:12702485-Receptors, Angiotensin, pubmed-meshheading:12702485-Renin-Angiotensin System, pubmed-meshheading:12702485-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:12702485-Signal Transduction, pubmed-meshheading:12702485-Vasoconstrictor Agents, pubmed-meshheading:12702485-Vasodilation
pubmed:year
2003
pubmed:articleTitle
Maturation-dependent changes of angiotensin receptor expression in fowl.
pubmed:affiliation
Dept. of Physiology, Univ. of Tennessee Health Science Center, 894 Union Ave., Memphis, TN 38163, USA. nishimur@physio1.utmem.edu
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't