Linked Life Data

12700196

Source:http://linkedlifedata.com/resource/pubmed/id/12700196

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2003-7-22
pubmed:abstractText
An understanding of testicular physiology and pathology requires knowledge of the regulation of cell death. Previous observation of suppression of apoptosis by hypoxia suggested a role for ATP in germ cell death. However, the exact effects of ATP production on germ cell death and of apoptosis on the levels of ATP and other adenine nucleotides (ANs) have remained unclear. We investigated the levels of ANs during human testicular apoptosis (analyzed by HPLC) and the role of chemical anoxia in germ cell death (detected by Southern blot analysis of DNA fragmentation, in situ end labeling of DNA, and electron microscopy). Incubation of seminiferous tubule segments under serum-free conditions induced apoptosis and concomitantly decreased the levels of ANs. Chemical anoxia, induced with potassium cyanide (KCN), an inhibitor of mitochondrial respiration, dropped ATP levels further and suppressed apoptosis at 4 h. After 24 h, many of the testicular cells underwent delayed apoptosis despite ATP depletion. Some cells showed signs of necrosis or toxicity. The addition of 2-deoxyglucose, an antimetabolite of glycolysis, did not alter the results obtained with KCN alone, whereas a toxic concentration of hydrogen peroxide switched apoptosis to necrosis. In most of the testicular cells, mitochondrial respiration appears to play a crucial role in controlling primary cell death cascades. In the human testis, there seem to be secondary apoptotic pathways that do not require functional respiration (or ATP).
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0006-3363
pubmed:author
pubmed:issnType
Print
pubmed:volume
69
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
617-26
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:12700196-Adenosine Diphosphate, pubmed-meshheading:12700196-Adenosine Monophosphate, pubmed-meshheading:12700196-Adenosine Triphosphate, pubmed-meshheading:12700196-Aged, pubmed-meshheading:12700196-Antimetabolites, pubmed-meshheading:12700196-Apoptosis, pubmed-meshheading:12700196-Blotting, Southern, pubmed-meshheading:12700196-Cell Hypoxia, pubmed-meshheading:12700196-DNA Fragmentation, pubmed-meshheading:12700196-Deoxyglucose, pubmed-meshheading:12700196-Glycolysis, pubmed-meshheading:12700196-Humans, pubmed-meshheading:12700196-Hydrogen Peroxide, pubmed-meshheading:12700196-In Situ Nick-End Labeling, pubmed-meshheading:12700196-Male, pubmed-meshheading:12700196-Microscopy, Electron, pubmed-meshheading:12700196-Middle Aged, pubmed-meshheading:12700196-Oxygen Consumption, pubmed-meshheading:12700196-Potassium Cyanide, pubmed-meshheading:12700196-Prostatic Neoplasms, pubmed-meshheading:12700196-Pyruvic Acid, pubmed-meshheading:12700196-Spermatozoa, pubmed-meshheading:12700196-Testis
pubmed:year
2003
pubmed:articleTitle
Chemical anoxia delays germ cell apoptosis in the human testis.
pubmed:affiliation
Program for Developmental and Reproductive Biology, Biomedicum Helsinki, and Hospital for Children and Adolescents, University of Helsinki, FIN-00029 HUS Helsinki, Finland. krista.erkkila@hus.fi
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't