|
rdf:type |
|
|
lifeskim:mentions |
umls-concept:C0005767,
umls-concept:C0010453,
umls-concept:C0016053,
umls-concept:C0033268,
umls-concept:C0079189,
umls-concept:C0178719,
umls-concept:C0205307,
umls-concept:C0333348,
umls-concept:C0487602,
umls-concept:C0518656,
umls-concept:C0806987
|
|
pubmed:issue |
2
|
|
pubmed:dateCreated |
2003-4-17
|
|
pubmed:abstractText |
It has been proposed that cytokines play a role in the pathogenesis of chronic fatigue syndrome (CFS) and fibromyalgia syndrome (FMS). However, different studies have reported conflicting results using enzyme-linked immunosorbent assay or polymerase chain reaction to detect cytokines in these conditions. In the present study, for the first time, the production of inflammatory [interleukin (IL)-1alpha, IL-6, and TNF-alpha] and anti-inflammatory (IL-10) cytokines by CD14+ and CD14- peripheral blood mononuclear cells (PBMC) from chronic fatigue syndrome (CFS) and fibromyalgia syndrome (FMS) patients and sex- and age-matched normal subjects was investigated at the level of individual cells using the technique of intracellular cytokine staining and flow cytometry. Cultures were carried out in the presence of polymyxin B to inhibit the effect of endotoxins on cytokine production by monocytes. The mean intensity of fluorescence (MIF) and percentage of CD14+ (monocytes) and CD14- (lymphocytes) cytokine-producing mononuclear cells were comparable in patients and controls in either unstimulated or IFN-gamma-stimulated conditions. Our study indicates that dysregulation of cytokine production by circulating monocytes or non-monocytic cells (lymphocytes) is not a dominant factor in the pathogenesis of CFS/FMS.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/12699429-10451910,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12699429-10634215,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12699429-10707990,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12699429-10722257,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12699429-10765933,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12699429-11081114,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12699429-11224736,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12699429-11477278,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12699429-11591124,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12699429-11983645,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12699429-1659582,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12699429-1698311,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12699429-1873478,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12699429-1999813,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12699429-2306288,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12699429-6646795,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12699429-7632928,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12699429-7684419,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12699429-7898182,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12699429-8463991,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12699429-8568312,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12699429-9201656,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12699429-9369341,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12699429-9495605,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12699429-9500148
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
May
|
|
pubmed:issn |
0009-9104
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
132
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
360-5
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:12699429-Adolescent,
pubmed-meshheading:12699429-Adult,
pubmed-meshheading:12699429-Case-Control Studies,
pubmed-meshheading:12699429-Cytokines,
pubmed-meshheading:12699429-Fatigue Syndrome, Chronic,
pubmed-meshheading:12699429-Female,
pubmed-meshheading:12699429-Fibromyalgia,
pubmed-meshheading:12699429-Flow Cytometry,
pubmed-meshheading:12699429-Fluorescent Antibody Technique,
pubmed-meshheading:12699429-Humans,
pubmed-meshheading:12699429-Interleukin-1,
pubmed-meshheading:12699429-Interleukin-10,
pubmed-meshheading:12699429-Interleukin-6,
pubmed-meshheading:12699429-Leukocytes, Mononuclear,
pubmed-meshheading:12699429-Male,
pubmed-meshheading:12699429-Middle Aged,
pubmed-meshheading:12699429-Statistics, Nonparametric,
pubmed-meshheading:12699429-Tumor Necrosis Factor-alpha
|
|
pubmed:year |
2003
|
|
pubmed:articleTitle |
Normal production of inflammatory cytokines in chronic fatigue and fibromyalgia syndromes determined by intracellular cytokine staining in short-term cultured blood mononuclear cells.
|
|
pubmed:affiliation |
Division of Molecular and Clinical Immunology, School of Clinical Laboratory Sciences, University of Nottingham, Queen's Medical Centre, Nottingham, UK.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
