Linked Life Data

12698357

Source:http://linkedlifedata.com/resource/pubmed/id/12698357

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2003-5-21
pubmed:abstractText
Intraganglionic laminar endings (IGLEs) represent the major vagal afferent structures throughout the gastrointestinal tract. Previous ultrastructural investigations have revealed synaptic contacts of IGLEs on myenteric neurons. Thus, in addtion to functioning probably as mechanosensors, IGLEs may also synaptically influence myenteric neurons. In search of clues for potential transmitters in IGLEs, we investigated, by combined neuronal tracing and immunocytochemistry in the esophagus, the correlation between IGLEs and vesicular glutamate transporter 2 (VGLUT2), which is considered a reliable marker for glutamatergic neurons. In rat esophagus, IGLEs were immunostained with calretinin. In the mouse, anterograde wheat germ agglutinin/horseradish peroxidase (WGA-HRP) tracing from nodose ganglion was used in order to label esophageal IGLEs. Confocal laser scanning microscopy demonstrated that VGLUT2 immunoreactivity was highly colocalized with synaptophysin and that both calretinin and tyramide amplified WGA-HRP in rat and mouse esophagus, respectively. No colocalization was found with calcitonin gene-related peptide, a marker for spinal primary afferents. Thus, VGLUT2 is found in vagal afferent endings in the esophagus, suggesting that glutamate is contained in, and probably released from, synaptic vesicles previously described in IGLEs. Functional evidence pending, this finding is in favor of a local effector function of IGLEs onto myenteric neurons.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0302-766X
pubmed:author
pubmed:issnType
Print
pubmed:volume
312
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
141-8
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:12698357-Animals, pubmed-meshheading:12698357-Carrier Proteins, pubmed-meshheading:12698357-Esophagus, pubmed-meshheading:12698357-Image Processing, Computer-Assisted, pubmed-meshheading:12698357-Immunohistochemistry, pubmed-meshheading:12698357-Mechanoreceptors, pubmed-meshheading:12698357-Membrane Transport Proteins, pubmed-meshheading:12698357-Mice, pubmed-meshheading:12698357-Mice, Inbred C57BL, pubmed-meshheading:12698357-Microscopy, Confocal, pubmed-meshheading:12698357-Neurotransmitter Agents, pubmed-meshheading:12698357-Nodose Ganglion, pubmed-meshheading:12698357-Rats, pubmed-meshheading:12698357-Vagus Nerve, pubmed-meshheading:12698357-Vesicular Glutamate Transport Protein 2, pubmed-meshheading:12698357-Vesicular Transport Proteins, pubmed-meshheading:12698357-Wheat Germ Agglutinin-Horseradish Peroxidase Conjugate
pubmed:year
2003
pubmed:articleTitle
Vesicular glutamate transporter 2 immunoreactivity in putative vagal mechanosensor terminals of mouse and rat esophagus: indication of a local effector function?
pubmed:affiliation
Institut für Anatomie, Lehrstuhl I, Universität Erlangen-Nürnberg, Krankenhausstrasse 9, 91054 Erlangen, Germany.
pubmed:publicationType
Journal Article, Comparative Study