Source:http://linkedlifedata.com/resource/pubmed/id/12697769
Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
26
|
pubmed:dateCreated |
2003-6-23
|
pubmed:databankReference | |
pubmed:abstractText |
Previously we reported that ferritin in corneal epithelial (CE) cells is a nuclear protein that protects DNA from UV damage. Since ferritin is normally cytoplasmic, in CE cells, a mechanism must exist that effects its nuclear localization. We have now determined that this involves a nuclear transport molecule we have termed ferritoid. Ferritoid is specific for CE cells and is developmentally regulated. Structurally, ferritoid contains multiple domains, including a functional SV40-type nuclear localization signal and a ferritin-like region of approximately 50% similarity to ferritin itself. This latter domain is likely responsible for the interaction between ferritoid and ferritin detected by co-immunoprecipitation analysis. To test functionally whether ferritoid is capable of transporting ferritin into the nucleus, we performed cotransfections of COS-1 cells with constructs for ferritoid and ferritin. Consistent with the proposed nuclear transport function for ferritoid, co-transfections with full-length constructs for ferritoid and ferritin resulted in a preferential nuclear localization of both molecules; this was not observed when the nuclear localization signal of ferritoid was deleted. Moreover, since ferritoid is structurally similar to ferritin, it may be an example of a nuclear transporter that evolved from the molecule it transports (ferritin).
|
pubmed:grant | |
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/Ferritins,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Transport Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Localization Signals,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins
|
pubmed:status |
MEDLINE
|
pubmed:month |
Jun
|
pubmed:issn |
0021-9258
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:day |
27
|
pubmed:volume |
278
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
23963-70
|
pubmed:dateRevised |
2007-11-14
|
pubmed:meshHeading |
pubmed-meshheading:12697769-Active Transport, Cell Nucleus,
pubmed-meshheading:12697769-Animals,
pubmed-meshheading:12697769-Base Sequence,
pubmed-meshheading:12697769-COS Cells,
pubmed-meshheading:12697769-Carrier Proteins,
pubmed-meshheading:12697769-Chick Embryo,
pubmed-meshheading:12697769-DNA, Complementary,
pubmed-meshheading:12697769-Epithelium, Corneal,
pubmed-meshheading:12697769-Ferritins,
pubmed-meshheading:12697769-Membrane Transport Proteins,
pubmed-meshheading:12697769-Molecular Sequence Data,
pubmed-meshheading:12697769-Nuclear Localization Signals,
pubmed-meshheading:12697769-Nuclear Proteins,
pubmed-meshheading:12697769-Protein Binding,
pubmed-meshheading:12697769-Protein Structure, Tertiary,
pubmed-meshheading:12697769-Transfection
|
pubmed:year |
2003
|
pubmed:articleTitle |
Ferritoid, a tissue-specific nuclear transport protein for ferritin in corneal epithelial cells.
|
pubmed:affiliation |
Department of Anatomy and Cellular Biology, Tufts University Medical School, Boston, Massachusetts 02111, USA.
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|