Source:http://linkedlifedata.com/resource/pubmed/id/12696121
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
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| pubmed:dateCreated |
2003-4-15
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| pubmed:abstractText |
The ability to vaccinate against polyomavirus infection in a T-cell deficient as well as a normal immune context was studied using polyomavirus major capsid protein (VP1) pseudocapsids (VP1-ps) or a glutathione-S-transferase-VP1 (GST-VP1) fusion protein. VP1-ps (1 or 10 microg) were administered subcutaneously, alone or together with Freund's complete and incomplete adjuvant, to CD4(-/-)8(-/-) T-cell deficient or normal C57Bl/6 mice on four occasions. Alternatively, CD4(-/-)8(-/-) and normal mice were inoculated with either GST-VP1 or Py-VP1-ps (5 microg). Following immunisation, antibody titres were tested by ELISA to VP1-ps or GST-VP1 or by haemagglutination inhibition (HAI). Mice were then infected with polyomavirus. Three weeks post-infection, the mice were killed and examined for the presence of polyomavirus DNA by PCR. Viral DNA was not detected in CD4(-/-)8(-/-) mice immunised with either VP1-ps alone or in combination with Freund's complete and incomplete adjuvant, or in any of the normal mice immunised with VP1-ps or GST-VP1. However, viral DNA was detected in 2/5 of the CD4(-/-)8(-/-) mice immunised with GST-VP1 and in non-immunised controls. Greater antibody titres were observed to VP1-ps than to GST-VP1 in CD4(-/-)8(-/-) mice after VP1-ps compared to GST-VP1 immunisation and antibody responses were better in normal than in immune-deficient mice. Only immunisation with VP1-ps resulted in haemagglutination inhibition. Complete protection against polyomavirus infection in the T-cell deficient context was obtained with VP1-ps, but not with GST-VP1, immunisation using the present vaccination protocol.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/Capsid Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Glutathione Transferase,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/VP1 protein, polyomavirus,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Vaccines
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| pubmed:status |
MEDLINE
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| pubmed:month |
Jun
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| pubmed:issn |
0146-6615
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| pubmed:author | |
| pubmed:copyrightInfo |
Copyright 2003 Wiley-Liss, Inc.
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| pubmed:issnType |
Print
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| pubmed:volume |
70
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
293-300
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:12696121-Animals,
pubmed-meshheading:12696121-Antibodies, Viral,
pubmed-meshheading:12696121-Capsid,
pubmed-meshheading:12696121-Capsid Proteins,
pubmed-meshheading:12696121-Disease Models, Animal,
pubmed-meshheading:12696121-Glutathione Transferase,
pubmed-meshheading:12696121-Guinea Pigs,
pubmed-meshheading:12696121-Hemagglutination,
pubmed-meshheading:12696121-Humans,
pubmed-meshheading:12696121-Immunization,
pubmed-meshheading:12696121-Immunologic Deficiency Syndromes,
pubmed-meshheading:12696121-Mice,
pubmed-meshheading:12696121-Mice, Inbred C57BL,
pubmed-meshheading:12696121-Polyomavirus,
pubmed-meshheading:12696121-Polyomavirus Infections,
pubmed-meshheading:12696121-Recombinant Fusion Proteins,
pubmed-meshheading:12696121-T-Lymphocytes,
pubmed-meshheading:12696121-Tumor Virus Infections,
pubmed-meshheading:12696121-Viral Vaccines
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| pubmed:year |
2003
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| pubmed:articleTitle |
VP1 pseudocapsids, but not a glutathione-S-transferase VP1 fusion protein, prevent polyomavirus infection in a T-cell immune deficient experimental mouse model.
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| pubmed:affiliation |
Department of Oncology-Pathology, Karolinska Institute, Karolinska Hospital, SE-171 76 Stockholm, Sweden. Andrea.Vlastos@cck.ki.se
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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