Source:http://linkedlifedata.com/resource/pubmed/id/12694393
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
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| pubmed:dateCreated |
2003-4-15
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| pubmed:databankReference | |
| pubmed:abstractText |
Many changes in neuronal gene expression occur in response to ischemia, and these may play a role in determining the fate of ischemic neurons. To identify genes induced in the rat brain following cerebral ischemia, a strategy was used that combines subtractive hybridization and differential screening. Among the genes identified was one referred to as global ischemia-inducible gene 11(Giig11). Sequence analysis indicated that Giig11 exhibited 97% and 91% identity to the known Ero1-L (S. cereviseae ero1-like oxidoreductase) of mouse and human origin, which is involved in oxidative endoplasmic reticulum protein folding. Rat Ero1-L/Giig11 also contains a l07-bp sequence that is nearly identical (> 95%) to the known dispersed repetitive identifier (ID), but which is lacking in mouse and human Ero1-L. Northern blotting showed that expression of the ID element and Ero1-L/Giig11 mRNA increased after global cerebral ischemia. In situ hybridization demonstrated increased expression of Ero1-L/Giig11 in the brain following ischemic injury, with the highest levels in the vulnerable hippocampal CA1 pyramidal neurons. Transfection of cultured primary hippocampal neurons with a plasmid containing green fluorescent protein (gfp) and Ero1-L/Giig11 cDNA (with and without the ID element) produced a gfp-Ero1-L/Giig11 fusion protein, and more fusion protein was localized into dendrites in the presence of the ID element, suggesting that the ID element promotes Ero1-L/Giig11 protein localization to dendrites. Therefore, Ero-1L/Giig11 may have a role in ischemia-induced neuronal repair or survival mechanisms directed at counteracting abnormalities in protein folding, maturation and distribution.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
May
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| pubmed:issn |
0022-3042
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| pubmed:author |
pubmed-author:GrahamSteven HSH,
pubmed-author:GreenbergDavid ADA,
pubmed-author:JinKunlinK,
pubmed-author:KawaguchiKenjiK,
pubmed-author:MaoXiao OuXO,
pubmed-author:NakayamaMasakiM,
pubmed-author:SimonRoger PRP,
pubmed-author:VanS LSL,
pubmed-author:XiongZhigangZ,
pubmed-author:ZhouAnA,
pubmed-author:ZhouXiaomanX
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| pubmed:issnType |
Print
|
| pubmed:volume |
85
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
670-9
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| pubmed:dateRevised |
2007-11-14
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| pubmed:meshHeading |
pubmed-meshheading:12694393-Amino Acid Sequence,
pubmed-meshheading:12694393-Animals,
pubmed-meshheading:12694393-Base Sequence,
pubmed-meshheading:12694393-Brain,
pubmed-meshheading:12694393-Brain Ischemia,
pubmed-meshheading:12694393-Cells, Cultured,
pubmed-meshheading:12694393-Dendrites,
pubmed-meshheading:12694393-Disease Models, Animal,
pubmed-meshheading:12694393-Gene Expression Regulation,
pubmed-meshheading:12694393-In Situ Hybridization,
pubmed-meshheading:12694393-Interspersed Repetitive Sequences,
pubmed-meshheading:12694393-Male,
pubmed-meshheading:12694393-Membrane Glycoproteins,
pubmed-meshheading:12694393-Mice,
pubmed-meshheading:12694393-Molecular Sequence Data,
pubmed-meshheading:12694393-Nerve Tissue Proteins,
pubmed-meshheading:12694393-Neurons,
pubmed-meshheading:12694393-RNA, Messenger,
pubmed-meshheading:12694393-Rats,
pubmed-meshheading:12694393-Sequence Analysis, DNA,
pubmed-meshheading:12694393-Sequence Homology
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| pubmed:year |
2003
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| pubmed:articleTitle |
Ero1-L, an ischemia-inducible gene from rat brain with homology to global ischemia-induced gene 11 (Giig11), is localized to neuronal dendrites by a dispersed identifier (ID) element-dependent mechanism.
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| pubmed:affiliation |
R. S. Dow Neurobiology Laboratories, Legacy Research, Portland, Oregon 97232, USA.
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|