Source:http://linkedlifedata.com/resource/pubmed/id/12693666
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
2003-4-15
|
| pubmed:abstractText |
Hepatocyte transplantation is emerging as an additional modality of treatment for patients with acute liver failure or liver-based metabolic disorders. The procedure requires isolation of high-quality hepatocytes from unused donor livers. Hepatocytes were isolated from 20 donor livers (11 right lobes, 3 left lateral segments, 6 whole livers) using a collagenase perfusion technique. Cell viability (median 56%, range 13-95%) and yield (median 1.4 x 10(9) cells, range 2.0 x 10(6)-1.8 x 10(10) cells) varied according to the tissue available. Fatty livers rejected for transplantation gave lower cell viability (median 45%, range 25-59%). There was a significant correlation between age of donor (median 21 years, range 7-66 years) and viability of isolated hepatocytes in vitro (r = -0.683, p = 0.001). The 13 segments of livers were from reduced/split grafts used for clinical transplantation in 9 children and 4 adults. There was no significant correlation between in vitro cell viability and clinical parameters including intensive care stay, serum aspartate aminotransferase,and international normalized ratio (in the first 7 days), and allograft rejection or other early posttransplant complications, in patients transplanted with the corresponding tissue.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0963-6897
|
| pubmed:author |
pubmed-author:AwMarion MMM,
pubmed-author:DhawanAnilA,
pubmed-author:GirlandaRaffaeleR,
pubmed-author:HeatonNigel DND,
pubmed-author:HughesRobin DRD,
pubmed-author:Mieli-VerganiGiorginaG,
pubmed-author:MitryRagai RRR,
pubmed-author:MuiesanPaoloP,
pubmed-author:RelaMohamedM,
pubmed-author:TerryClaireC
|
| pubmed:issnType |
Print
|
| pubmed:volume |
12
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
69-74
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:12693666-Adolescent,
pubmed-meshheading:12693666-Adult,
pubmed-meshheading:12693666-Age Factors,
pubmed-meshheading:12693666-Aged,
pubmed-meshheading:12693666-Aspartate Aminotransferases,
pubmed-meshheading:12693666-Cell Count,
pubmed-meshheading:12693666-Cell Culture Techniques,
pubmed-meshheading:12693666-Cell Separation,
pubmed-meshheading:12693666-Cell Survival,
pubmed-meshheading:12693666-Child,
pubmed-meshheading:12693666-Female,
pubmed-meshheading:12693666-Graft Rejection,
pubmed-meshheading:12693666-Graft Survival,
pubmed-meshheading:12693666-Hepatocytes,
pubmed-meshheading:12693666-Humans,
pubmed-meshheading:12693666-Liver Failure, Acute,
pubmed-meshheading:12693666-Male,
pubmed-meshheading:12693666-Middle Aged,
pubmed-meshheading:12693666-Tissue Transplantation
|
| pubmed:year |
2003
|
| pubmed:articleTitle |
Human hepatocyte isolation and relationship of cell viability to early graft function.
|
| pubmed:affiliation |
Institute of Liver Studies, Guy's, King's and St. Thomas' School of Medicine, and King's College Hospital, Denmark Hill, London, UK.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|