Source:http://linkedlifedata.com/resource/pubmed/id/12691822
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
2003-4-14
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| pubmed:abstractText |
Frequent observations of allelic loss in chromosomal band 17q25.1 in a variety of human cancers have suggested that one or more tumor suppressor genes are normally present in this region. Moreover, a locus responsible for hereditary focal non-epidermolytic palmoplantar keratoderma (tylosis oesophageal cancer; TOC), a condition associated with esophageal cancer, has been mapped to the same band. During efforts to sequence, by shot-gun methods, a 1 Mb target region that we had defined as the DNA segment harboring the putative tumor suppressor gene(s) involved in these events, we identified a novel cDNA, DRHC (down-regulated in human cancers), that showed reduced expression in 28 of 95 (29%) cell lines derived from a variety of human cancers. The full-length cDNA, 6275 bp long, was expressed predominantly in thymus and brain. The predicted 1942-amino-acid product exhibited significant sequence homology to yeast enzymes belonging to the DEAD-helicase superfamily, and appeared to be a Uvr/Rep helicase with a DEXDc consensus domain. Transfection of a DRHC expression vector inhibited growth of cancer cells in liquid medium or soft agar. The results suggest that loss of expression of DRHC may play a role in human carcinogenesis.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Apr
|
| pubmed:issn |
0304-3835
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
10
|
| pubmed:volume |
193
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
41-7
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| pubmed:dateRevised |
2007-11-15
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| pubmed:meshHeading |
pubmed-meshheading:12691822-Blotting, Northern,
pubmed-meshheading:12691822-Brain,
pubmed-meshheading:12691822-Cell Division,
pubmed-meshheading:12691822-Chromosomes, Human, Pair 17,
pubmed-meshheading:12691822-Cloning, Molecular,
pubmed-meshheading:12691822-DNA,
pubmed-meshheading:12691822-DNA, Complementary,
pubmed-meshheading:12691822-DNA Helicases,
pubmed-meshheading:12691822-Databases as Topic,
pubmed-meshheading:12691822-Down-Regulation,
pubmed-meshheading:12691822-Esophageal Neoplasms,
pubmed-meshheading:12691822-Genes, Tumor Suppressor,
pubmed-meshheading:12691822-Genetic Vectors,
pubmed-meshheading:12691822-Humans,
pubmed-meshheading:12691822-Models, Genetic,
pubmed-meshheading:12691822-Neoplasm Proteins,
pubmed-meshheading:12691822-Neoplasms,
pubmed-meshheading:12691822-Polymorphism, Single-Stranded Conformational,
pubmed-meshheading:12691822-Protein Structure, Tertiary,
pubmed-meshheading:12691822-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:12691822-Thymus Gland,
pubmed-meshheading:12691822-Time Factors,
pubmed-meshheading:12691822-Transfection,
pubmed-meshheading:12691822-Tumor Cells, Cultured
|
| pubmed:year |
2003
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| pubmed:articleTitle |
Down-regulation in human cancers of DRHC, a novel helicase-like gene from 17q25.1 that inhibits cell growth.
|
| pubmed:affiliation |
Department of Molecular Biology, Institute of Gerontology, Nippon Medical School. 1-396, Kosugi-cho, Nakahara-ku, Kawasaki 211-0063, Japan.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|