Linked Life Data

12687708

Source:http://linkedlifedata.com/resource/pubmed/id/12687708

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2003-4-10
pubmed:abstractText
Gap junctions regulate a variety of cell functions by directly connecting two cells through intercellular channels. Connexins are gap junction channel-forming protein subunits. In this study, we studied the expression of connexin 36 (Cx36) in the olfactory epithelium and olfactory bulb of adult mice. In situ hybridization revealed that mRNA for Cx36 was expressed in the olfactory sensory epithelium, main olfactory bulb and accessory olfactory bulb. Expression of mRNA encoding Cx36 was observed in the olfactory epithelium mainly in ventral and lateral regions of the turbinates. Immunohistochemical determination of Cx36 protein expression showed sparse punctuate staining in the olfactory epithelial layer. Intense Cx36-like immunostaining was found in the olfactory nerve bundles underlying the olfactory epithelium and in the olfactory nerve layer and glomerular layer of the olfactory bulb. Mapping of the intensity of Cx36-like immunofluorescence in glomeruli throughout the main olfactory bulb indicated a heterogeneous distribution. A set of approximately 50 glomeruli located in the anterior and posterior limits of the olfactory bulb was more intensely labeled than other glomeruli. There was intense immunofluorescence signal in the glomerular layer of the accessory olfactory bulb and in the vomeronasal nerve. beta-Galactosidase distribution in the olfactory epithelium and olfactory bulb in Cx36 knockout mice (Deans et al. [2001] Neuron 31:477-485) supported the findings with immunofluorescence. Cx36-like immunofluorescence was absent in the olfactory nerve bundles in Cx36 knockout mice. The immunolocalization of Cx36 to the olfactory and vomeronasal nerves, and a subset of olfactory glomeruli suggest a functional role for Cx36 in odor coding.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0021-9967
pubmed:author
pubmed:copyrightInfo
Copyright 2003 Wiley-Liss, Inc.
pubmed:issnType
Print
pubmed:day
12
pubmed:volume
459
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
426-39
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
Heterogeneous expression of connexin 36 in the olfactory epithelium and glomerular layer of the olfactory bulb.
pubmed:affiliation
Department of Cellular and Structural Biology, the Rocky Mountain Taste and Smell Center and Neuroscience Program, University of Colorado Health Sciences Center, Denver 80262, USA. chunbo.zhang@uchsc.edu
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.