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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2003-7-9
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pubmed:abstractText |
CCK-58 differs from CCK-8 in patterns of expression of pancreatic secretion of fluid and amylase and gallbladder contraction. These differences have physiological relevance only if CCK-58 release is stimulated by nutrients entering the intestine and if CCK-58 circulates in sizeable amounts. In this study, we report that when radiolabeled CCK-58 is added to rat blood and plasma is formed, there is extensive loss and degradation of the radioactive peptide. Therefore, a new method was developed to minimize loss and degradation of this label. This method recovered >85% of the label with no detectable degradation. Furthermore, the optimized method recovered all unlabeled exogenous cholecystokinin molecular forms in >80% yields. Blood from fasted rats and rats in which cholecystokinin release was stimulated by the trypsin inhibitor camostat contained only CCK-58 (3.5 +/- 0.5 and 17 +/- 1.5 fmol/ml, respectively). Because CCK-58 predominates in the blood, this molecular form should be used in studies on the physiology and pathophysiology of cholecystokinin.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Buffers,
http://linkedlifedata.com/resource/pubmed/chemical/Cholecystokinin,
http://linkedlifedata.com/resource/pubmed/chemical/FOY 305,
http://linkedlifedata.com/resource/pubmed/chemical/Gabexate,
http://linkedlifedata.com/resource/pubmed/chemical/Iodine Radioisotopes,
http://linkedlifedata.com/resource/pubmed/chemical/Peptide Fragments,
http://linkedlifedata.com/resource/pubmed/chemical/Plant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Sincalide,
http://linkedlifedata.com/resource/pubmed/chemical/Trypsin Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Tyrosine,
http://linkedlifedata.com/resource/pubmed/chemical/alpha-Amylases,
http://linkedlifedata.com/resource/pubmed/chemical/cholecystokinin 22 C-terminal...,
http://linkedlifedata.com/resource/pubmed/chemical/cholecystokinin 58
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0193-1857
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
285
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
G255-65
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:12686511-Amino Acid Sequence,
pubmed-meshheading:12686511-Animals,
pubmed-meshheading:12686511-Buffers,
pubmed-meshheading:12686511-Cholecystokinin,
pubmed-meshheading:12686511-Chromatography, High Pressure Liquid,
pubmed-meshheading:12686511-Gabexate,
pubmed-meshheading:12686511-Hydrogen-Ion Concentration,
pubmed-meshheading:12686511-Iodine Radioisotopes,
pubmed-meshheading:12686511-Isotope Labeling,
pubmed-meshheading:12686511-Molecular Sequence Data,
pubmed-meshheading:12686511-Peptide Fragments,
pubmed-meshheading:12686511-Plant Proteins,
pubmed-meshheading:12686511-Rats,
pubmed-meshheading:12686511-Sincalide,
pubmed-meshheading:12686511-Trypsin Inhibitors,
pubmed-meshheading:12686511-Tyrosine,
pubmed-meshheading:12686511-alpha-Amylases
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pubmed:year |
2003
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pubmed:articleTitle |
CCK-58 is the only detectable endocrine form of cholecystokinin in rat.
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pubmed:affiliation |
Center for Ulcer Research and Education, Veterans Affairs Greater Los Angeles Healthcare System, 90073, USA. jreeve@ucla.edu
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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