Source:http://linkedlifedata.com/resource/pubmed/id/12684813
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
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| pubmed:dateCreated |
2003-5-7
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| pubmed:abstractText |
Transforming growth factor beta 1 (TGF-beta 1) contributes to the accumulation of extracellular matrix (ECM) in the tubulointerstitial space in chronic renal diseases. Identification of target cells and the contribution of epithelial-mesenchymal transformation (EMT) in TGF-beta 1-induced fibrosis in vivo are currently under investigation. We have developed a transgenic model of slowly developing TGF-beta 1-driven tubulointerstitial fibrosis (TIF). By using this model our aim was to localise the ECM-producing cells, to investigate the temporal and spatial distribution of the cellular markers alpha-smooth muscle cell actin (alpha SM-actin), Fsp1 and Hsp47 and to explore the possible involvement of EMT in TGF-beta1-induced TIF in vivo. We utilised a combination of in situ hybridisation, immunohistochemistry and western blotting techniques and found that alpha SM-actin-positive interstitial cells are the main source of collagen types I and III and fibronectin, whereas collagen type IV(alpha 1/alpha 2) originates mainly from the tubular epithelial cells. Furthermore, macrophages are not important combatants during the early course of TGF-beta 1-induced TIF. Finally, EMT is not necessary for the initiation of TGF-beta 1-induced TIF. We conclude, that intervention directed against the recruitment of activated interstitial cells may avoid the development of end-stage renal disease.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Biological Markers,
http://linkedlifedata.com/resource/pubmed/chemical/Collagen,
http://linkedlifedata.com/resource/pubmed/chemical/Fibronectins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Tgfb1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Transforming Growth Factor beta,
http://linkedlifedata.com/resource/pubmed/chemical/Transforming Growth Factor beta1
|
| pubmed:status |
MEDLINE
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| pubmed:month |
Apr
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| pubmed:issn |
0948-6143
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
119
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
267-80
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:12684813-Animals,
pubmed-meshheading:12684813-Biological Markers,
pubmed-meshheading:12684813-Blotting, Western,
pubmed-meshheading:12684813-Collagen,
pubmed-meshheading:12684813-Disease Models, Animal,
pubmed-meshheading:12684813-Extracellular Matrix,
pubmed-meshheading:12684813-Female,
pubmed-meshheading:12684813-Fibronectins,
pubmed-meshheading:12684813-Fibrosis,
pubmed-meshheading:12684813-Immunohistochemistry,
pubmed-meshheading:12684813-In Situ Hybridization,
pubmed-meshheading:12684813-Male,
pubmed-meshheading:12684813-Mice,
pubmed-meshheading:12684813-Mice, Inbred BALB C,
pubmed-meshheading:12684813-Mice, Transgenic,
pubmed-meshheading:12684813-Nephritis, Interstitial,
pubmed-meshheading:12684813-Phenotype,
pubmed-meshheading:12684813-RNA, Messenger,
pubmed-meshheading:12684813-Transforming Growth Factor beta,
pubmed-meshheading:12684813-Transforming Growth Factor beta1
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| pubmed:year |
2003
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| pubmed:articleTitle |
Localisation and phenotypical characterisation of collagen-producing cells in TGF-beta 1-induced renal interstitial fibrosis.
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| pubmed:affiliation |
The Research Laboratory for Biochemical Pathology, The Institute for Experimental Clinical Research, Aarhus Kommunehospital, 44-Noerrebrogade, 8000 Aarhus C, Denmark.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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