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PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2003-4-9
pubmed:abstractText
Using in vitro and in silico approaches, we demonstrate that the Hmob3 brain-specific sequence is a part of the extended human MAP1B gene 3'UTR encoded by the portion of the exon 7. Previously we had isolated the 1.4 kb Hmob3 clone (Acc. No Y09836) from the human medulla oblongata cDNA library. In the present paper, we report the RT-PCR detection of extended transcripts containing the Hmob3 sequence as well as a fragment of MAP1B mRNA in the human brain mRNA samples. The existence of such transcripts confirms that Hmob3 is transcribed from the MAP1B gene. Thus we report a previously unknown region of the human MAP1B gene which encodes the MAP1B mRNA 3'UTR. The length of the 3'UTR was estimated of 4330 nucleotides. Comparative sequence analysis shows high phylogenetic conservation of MAP1B 3'UTR in mammals. Using Hmob3 as a probe in the Northern blot analysis, we have revealed two different types of transcripts for brain and kidney mRNA samples; in the skeletal muscle sample we detected both transcripts.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
1389-0344
pubmed:author
pubmed:issnType
Print
pubmed:volume
20
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
91-6
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
Hmob3 brain-specific sequence is a part of phylogenetically conserved human MAP1B gene 3'-untranslated region.
pubmed:affiliation
Institute of Molecular Genetics, Russian Academy of Sciences, Moscow 123182, Russia. dergunov@img.ras.ru
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't