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rdf:type |
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lifeskim:mentions |
umls-concept:C0007595,
umls-concept:C0009015,
umls-concept:C0025831,
umls-concept:C0033684,
umls-concept:C0035711,
umls-concept:C0039476,
umls-concept:C0085475,
umls-concept:C1514873,
umls-concept:C1537998,
umls-concept:C1546857,
umls-concept:C1556066,
umls-concept:C1619636,
umls-concept:C1880022,
umls-concept:C2248741
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pubmed:issue |
8
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pubmed:dateCreated |
2003-4-8
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pubmed:databankReference |
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pubmed:abstractText |
N1-methyladenosine (m1A) is found at position 58 in the T-loop of many tRNAs. In yeast, the formation of this modified nucleoside is catalyzed by the essential tRNA (m1A58) methyltransferase, a tetrameric enzyme that is composed of two types of subunits (Gcd14p and Gcd10p). In this report we describe the cloning, expression and characterization of a Gcd14p homolog from the hyperthermophilic bacterium Thermus thermophilus. The purified recombinant enzyme behaves as a homotetramer of 150 kDa by gel filtration and catalyzes the site- specific formation of m1A at position 58 of the T-loop of tRNA in the absence of any other complementary protein. S-adenosylmethionine is used as donor of the methyl group. Thus, we propose to name the bacterial enzyme TrmI and accordingly its structural gene trmI. These results provide a key evolutionary link between the functionally characterized two-component eukaryotic enzyme and the recently described crystal structure of an uncharacterized, putative homotetrameric methyltransferase Rv2118c from Mycobacterium tuberculosis. Interest ingly, inactivation of the T.thermophilus trmI gene results in a thermosensitive phenotype (growth defect at 80 degrees C), which suggests a role of the N1-methylation of tRNA adenosine-58 in adaptation of life to extreme temperatures.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/12682365-10330157,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12682365-10550050,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12682365-10561594,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12682365-10748051,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12682365-10779558,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12682365-11554794,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12682365-11684083,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12682365-11927565,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12682365-12110595,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12682365-12403464,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12682365-1289795,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12682365-14292146,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12682365-1625584,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12682365-1742347,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12682365-17605,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12682365-220499,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12682365-2449804,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12682365-2722737,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12682365-2823642,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12682365-3003740,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12682365-3277187,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12682365-3769915,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12682365-3853464,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12682365-3957870,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12682365-4604768,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12682365-4881901,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12682365-6190418,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12682365-7151806,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12682365-7516708,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12682365-7599271,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12682365-7692402,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12682365-8230205,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12682365-9254694,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12682365-9399820,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12682365-9399834,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12682365-9671058,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12682365-9851972,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12682365-985514
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
1362-4962
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
15
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pubmed:volume |
31
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2148-56
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:12682365-Amino Acid Sequence,
pubmed-meshheading:12682365-Cell Division,
pubmed-meshheading:12682365-Chromatography, Gel,
pubmed-meshheading:12682365-Cloning, Molecular,
pubmed-meshheading:12682365-Dimerization,
pubmed-meshheading:12682365-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:12682365-Hot Temperature,
pubmed-meshheading:12682365-Molecular Sequence Data,
pubmed-meshheading:12682365-Mutation,
pubmed-meshheading:12682365-Phenotype,
pubmed-meshheading:12682365-RNA, Transfer, Asp,
pubmed-meshheading:12682365-Sequence Homology, Amino Acid,
pubmed-meshheading:12682365-Temperature,
pubmed-meshheading:12682365-Thermus thermophilus,
pubmed-meshheading:12682365-tRNA Methyltransferases
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pubmed:year |
2003
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pubmed:articleTitle |
Cloning and characterization of tRNA (m1A58) methyltransferase (TrmI) from Thermus thermophilus HB27, a protein required for cell growth at extreme temperatures.
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pubmed:affiliation |
Laboratoire de Microbiologie, Université Libre de Bruxelles, Institut de Recherches Microbiologiques Jean-Marie Wiame, Avenue E. Gryson 1, B-1070 Bruxelles, Belgium.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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