Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2003-4-8
pubmed:abstractText
Abstract Epidermal growth factor (EGF) and receptor (-R) signaling pathway is required for epithelial cell growth and differentiation such as the degeneration of the medial edge epithelial cells during the fusion process of secondary palate formation. As epithelial fusion takes place during primary palate formation, we investigated the involvement of the EGF-R in fusion of the medial (MNP) and lateral (LNP) nasal prominences of the mouse embryo was examined. Immunoreactivity of EGF-R was investigated in embryonic day 10 embryos (32-37 somite stages). The EGF-R immunoreactivity was observed in the nasal epithelia of the presumptive fusion area before fusion. It became undetectable just prior to the fusion and faintly reappeared at the time of the fusion. In contrast, the non-fusing epithelial cells of the nasal groove maintained the immunoreactivity throughout these stages. In order to elucidate whether the EGF/EGF-R signaling pathway was involved in nasal epithelial fusion, EGF solution was injected into the exocoelum of explanted mouse embryos, and the embryos were cultured for 18-24 h by whole embryo culture (WEC). This exogenous EGF inhibited fusion of nasal prominences in 66.7-81.5% of the embryos. Treatment with EGF for 4-14 h showed that exogenous EGF disturbed the EGF-R disappearance and normal alteration of epithelial cell morphology in the fusion area. These results suggest that temporal disappearance of the EGF/EGF-R signaling from presumptive fusion of the nasal prominences is required for morphological change of the epithelial cells leading to the fusion of MNP and LNP.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
1447-6959
pubmed:author
pubmed:issnType
Print
pubmed:volume
78
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
25-35
pubmed:dateRevised
2011-2-8
pubmed:meshHeading
pubmed-meshheading:12680467-Animals, pubmed-meshheading:12680467-Body Patterning, pubmed-meshheading:12680467-Cell Differentiation, pubmed-meshheading:12680467-Dose-Response Relationship, Drug, pubmed-meshheading:12680467-Down-Regulation, pubmed-meshheading:12680467-Embryo, Mammalian, pubmed-meshheading:12680467-Epidermal Growth Factor, pubmed-meshheading:12680467-Female, pubmed-meshheading:12680467-Fetus, pubmed-meshheading:12680467-Gene Expression Regulation, Developmental, pubmed-meshheading:12680467-Immunohistochemistry, pubmed-meshheading:12680467-Male, pubmed-meshheading:12680467-Mice, pubmed-meshheading:12680467-Mice, Inbred C57BL, pubmed-meshheading:12680467-Microscopy, Electron, Scanning, pubmed-meshheading:12680467-Nasal Cavity, pubmed-meshheading:12680467-Nasal Mucosa, pubmed-meshheading:12680467-Organ Culture Techniques, pubmed-meshheading:12680467-Palate, pubmed-meshheading:12680467-Pregnancy, pubmed-meshheading:12680467-Receptor, Epidermal Growth Factor
pubmed:year
2003
pubmed:articleTitle
Disappearance of epidermal growth factor receptor is essential in the fusion of the nasal epithelium.
pubmed:affiliation
Section of Molecular Craniofacial Embryology, Graduate School, Tokyo Medical and Dental University, Yushima, Bunkyo-ku, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't