Linked Life Data

12676256

Source:http://linkedlifedata.com/resource/pubmed/id/12676256

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2003-4-4
pubmed:abstractText
Type I plasminogen activator inhibitor (PAI-1) is a 50 kDa glycoprotein belonging to the serine protease superfamily. PAI-1 is consistently and dramatically upregulated in a variety of fibrotic diseases. The aim of this study was to compare PAI-1 expression in normal human buccal mucosa and oral submucous fibrosis (OSF) specimens and further explore the potential mechanism that may lead to induced PAI-1 expression. Twenty-five OSF specimens and six normal buccal mucosa were examined by immunohistochemistry. The activity of PAI-1 from cells cultured from OSF and normal buccal mucosa were assayed using reverse-transcriptase polymerase chain reaction (RT-PCR) and Western blots. PAI-1 expression was significantly higher in OSF specimens and expressed mainly by fibroblasts, endothelial cells, and inflammatory cells. In addition, OSF exhibited higher PAI-1 expression than normal buccal mucosa fibroblast (BMF) both in mRNA and protein levels. To verify whether arecoline, a major areca nut alkaloid, could affect PAI-1 expression by human BMFs, RT-PCR and Western blots were used. The results demonstrated highly elevated PAI-1 mRNA and protein expression in normal human BMFs stimulated by arecoline. Taken together, these results suggest that PAI-1 expression is significantly upregulated in OSF tissues from areca quid chewers, and arecoline may be responsible for the enhanced PAI-1 expression in vivo.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1368-8375
pubmed:author
pubmed:copyrightInfo
Copyright 2003 Elsevier Science Ltd.
pubmed:issnType
Print
pubmed:volume
39
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
367-72
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
The upregulation of type I plasminogen activator inhibitor in oral submucous fibrosis.
pubmed:affiliation
Institute of Biochemistry, Chung Shan Medical University, Taichung, Taiwan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't