Source:http://linkedlifedata.com/resource/pubmed/id/12672076
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
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| pubmed:dateCreated |
2003-4-2
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| pubmed:abstractText |
Optimal proliferation of T cells, although initiated via ligation of the CD3/TCR complex, requires additional costimulatory signals. While the most well-defined in vitro pathway of costimulation is via the B7 family of molecules, a large body of data clearly demonstrates an in vivo role for the CD40/CD154 pathway in transplantation, autoimmunity and allergy. We have examined the role of CD40 as an independent costimulatory molecule by generating a panel of transfected human fibroblasts expressing DR1 with either CD80, CD86 or CD40. Functional assays using allogeneic CD4(+) T cells as responders demonstrated that CD40 was capable of costimulating CD4(+) T cell proliferation particularly in the CD45RO subset. Costimulation by CD40 induced much higher levels of IL-10 than were induced by B7-expressing cells. On day 3 the dominant costimulation was provided by CD40, while by day 5 this was overshadowed by CD80 and CD86. Nonetheless, the provision of costimulation by CD40 was enough to expand an alloreactive T cell line. These results were confirmed in experiments using primary cultures of CD40(+)CD80/CD86(-) renal tubular epithelial cells as the stimulator population. Thus, CD40 is capable of functioning independently (in the absence of B7) as a costimulatory molecule both in terms of proliferation and cytokine release. The data have interesting implications concerning the consequences of antigen presentation by tissue parenchymal cells.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD40,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD80,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD86,
http://linkedlifedata.com/resource/pubmed/chemical/CD86 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Cd86 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/HLA-DR1 Antigen,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-10,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Apr
|
| pubmed:issn |
0014-2980
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
33
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1094-104
|
| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:12672076-Animals,
pubmed-meshheading:12672076-Antigens, CD,
pubmed-meshheading:12672076-Antigens, CD40,
pubmed-meshheading:12672076-Antigens, CD80,
pubmed-meshheading:12672076-Antigens, CD86,
pubmed-meshheading:12672076-CD4-Positive T-Lymphocytes,
pubmed-meshheading:12672076-Cell Line,
pubmed-meshheading:12672076-Coculture Techniques,
pubmed-meshheading:12672076-Fibroblasts,
pubmed-meshheading:12672076-HLA-DR1 Antigen,
pubmed-meshheading:12672076-Humans,
pubmed-meshheading:12672076-Immunologic Memory,
pubmed-meshheading:12672076-Interleukin-10,
pubmed-meshheading:12672076-Kidney Tubules,
pubmed-meshheading:12672076-Kinetics,
pubmed-meshheading:12672076-Lymphocyte Activation,
pubmed-meshheading:12672076-Membrane Glycoproteins,
pubmed-meshheading:12672076-Mice,
pubmed-meshheading:12672076-Transfection
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| pubmed:year |
2003
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| pubmed:articleTitle |
CD40 can costimulate human memory T cells and favors IL-10 secretion.
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| pubmed:affiliation |
Department of Immunology, Imperial College of Science, Technology and Medicine, London, GB.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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