Linked Life Data

12670699

Source:http://linkedlifedata.com/resource/pubmed/id/12670699

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PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
2003-4-2
pubmed:abstractText
The alpha(2C)-adrenoceptor occurs in high density in the striatum relative to other brain regions, but its biological role in striatal physiology is perplexing because of the paucity of noradrenergic terminals in this region. In this study, mice with a targeted inactivation of the alpha(2C)-adrenoceptor gene (alpha(2C)-KO mice), and genetically related mice (WT mice), were used to study the potential role of the striatal alpha(2C)-adrenoceptor in modulating GABA release. Perfused brain slices were pre-loaded with [(3)H]GABA and were stimulated electrically. In WT mice, the alpha(2)-adrenoceptor agonist, UK14304 (brimonidine), significantly enhanced [(3)H]GABA release from striatal slices, while the alpha(2)-adrenoceptor antagonist, RX821002, alone evoked a significant decrease in [(3)H]GABA release. In alpha(2C)-KO mice, the effect of RX821002 was absent, while UK14304 retained its ability to enhance [(3)H]GABA release. Pharmacological depletion of monoamines in WT mice also abolished the effect of RX821002 on [(3)H]GABA release. In hippocampal slices, RX821002-induced reduction in [(3)H]GABA release was present in WT and alpha(2C)-KO mice. In the presence of tetrodotoxin, RX821002 increased [(3)H]GABA release in striatal slices from both WT and alpha(2C)-KO mice. Together, these data imply that alpha(2A)- and alpha(2C)-adrenoceptors are located on different neurons in the striatum, that alpha(2C)-adrenoceptor-mediated effects on striatal GABA release are mediated by an endogenous catecholamine that could be dopamine, and that the alpha(2C)-adrenoceptor effect of RX821002 does not occur at the GABAergic terminal.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0169-328X
pubmed:author
pubmed:issnType
Print
pubmed:day
10
pubmed:volume
112
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
24-32
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed-meshheading:12670699-Adrenergic alpha-Agonists, pubmed-meshheading:12670699-Adrenergic alpha-Antagonists, pubmed-meshheading:12670699-Animals, pubmed-meshheading:12670699-Electric Stimulation, pubmed-meshheading:12670699-Hippocampus, pubmed-meshheading:12670699-Male, pubmed-meshheading:12670699-Mice, pubmed-meshheading:12670699-Mice, Inbred C57BL, pubmed-meshheading:12670699-Mice, Knockout, pubmed-meshheading:12670699-Neostriatum, pubmed-meshheading:12670699-Norepinephrine, pubmed-meshheading:12670699-Organ Culture Techniques, pubmed-meshheading:12670699-Presynaptic Terminals, pubmed-meshheading:12670699-Receptors, Adrenergic, alpha-2, pubmed-meshheading:12670699-Synapses, pubmed-meshheading:12670699-Synaptic Transmission, pubmed-meshheading:12670699-Tetrodotoxin, pubmed-meshheading:12670699-Tritium, pubmed-meshheading:12670699-gamma-Aminobutyric Acid
pubmed:year
2003
pubmed:articleTitle
The alpha2C-adrenoceptor modulates GABA release in mouse striatum.
pubmed:affiliation
Departments of Psychiatry and Behavior and Pharmacology and Toxicology, University of Mississippi Medical Center, 2500 North State Street, Jackson, MS 39216, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't