|
rdf:type |
|
|
lifeskim:mentions |
umls-concept:C0007600,
umls-concept:C0016057,
umls-concept:C0021467,
umls-concept:C0021469,
umls-concept:C0031715,
umls-concept:C0056695,
umls-concept:C0162638,
umls-concept:C0220802,
umls-concept:C0332293,
umls-concept:C0441712,
umls-concept:C1366765,
umls-concept:C2349975
|
|
pubmed:issue |
3
|
|
pubmed:dateCreated |
2003-4-2
|
|
pubmed:abstractText |
We evaluated the role of MAPKs on apoptosis induced by butyrate in cells derived from a human fibrosarcoma (2C4). Culture of 2C4 cells in 5% of fetal bovine serum (FBS) induced ERK1/2 and CREB phosphorylation and delayed apoptosis induced by butyrate. Butyrate inhibited phosphorylation of ERK1/2 and CREB. Furthermore, the use of specific inhibitors PD98059 (MEK) and H89 (PKA), which block ERK1/2 and CREB phosphorylation, accelerated butyrate induced cell death in 2C4 cells. The butyrate effect was shown to be dependent on caspase activation, once caspase inhibitors restored phosphorylation of ERK1/2 and CREB in 2C4 cells. However, the proteolytic effect of caspases was not directly on ERK1/2 and CREB proteins. In conclusion, butyrate induced apoptosis in 2C4 cells is regulated by the levels of ERK1/2 and CREB phosphorylation in a caspase dependent mechanism.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antineoplastic Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Butyrates,
http://linkedlifedata.com/resource/pubmed/chemical/Caspases,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP Response...,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Flavonoids,
http://linkedlifedata.com/resource/pubmed/chemical/Isoquinolines,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinase 1,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinase 3,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/N-(2-(4-bromocinnamylamino)ethyl)-5-...,
http://linkedlifedata.com/resource/pubmed/chemical/PD 98059,
http://linkedlifedata.com/resource/pubmed/chemical/Sulfonamides
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Apr
|
|
pubmed:issn |
0006-291X
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
11
|
|
pubmed:volume |
303
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
968-72
|
|
pubmed:dateRevised |
2010-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:12670506-Antineoplastic Agents,
pubmed-meshheading:12670506-Apoptosis,
pubmed-meshheading:12670506-Butyrates,
pubmed-meshheading:12670506-Caspases,
pubmed-meshheading:12670506-Cyclic AMP Response Element-Binding Protein,
pubmed-meshheading:12670506-Enzyme Inhibitors,
pubmed-meshheading:12670506-Fibrosarcoma,
pubmed-meshheading:12670506-Flavonoids,
pubmed-meshheading:12670506-Humans,
pubmed-meshheading:12670506-Isoquinolines,
pubmed-meshheading:12670506-Mitogen-Activated Protein Kinase 1,
pubmed-meshheading:12670506-Mitogen-Activated Protein Kinase 3,
pubmed-meshheading:12670506-Mitogen-Activated Protein Kinases,
pubmed-meshheading:12670506-Phosphorylation,
pubmed-meshheading:12670506-Signal Transduction,
pubmed-meshheading:12670506-Sulfonamides,
pubmed-meshheading:12670506-Tumor Cells, Cultured
|
|
pubmed:year |
2003
|
|
pubmed:articleTitle |
Inhibition of ERK1/2 and CREB phosphorylation by caspase-dependent mechanism enhances apoptosis in a fibrosarcoma cell line treated with butyrate.
|
|
pubmed:affiliation |
Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Caixa Postal 486, CEP 30 161-970, Belo Horizonte, Minas Gerais, Brazil.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
