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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2003-4-2
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pubmed:abstractText |
This study investigated the effect of oleic acid on the expression levels of endothelin-1 (ET-1) and on the signaling pathways mediating it in human aortic endothelial cells (HAECs). ET-1 mRNA expression was significantly increased by oleic acid in a dose- and time-dependent manner. Elevation of ET-1 expression in response to oleic acid was inhibited by the protein kinase C (PKC) inhibitor, GF109203X, or the NF-kappa B inhibitor, pyrrolidine dithiocarbamate. In addition, both PKC and NF-kappa B activities were significantly increased by oleic acid. Immunoblot analysis revealed that conventional PKCs (PKC-alpha and -beta II isoforms) were significantly increased in the membranous fractions of HAECs treated with oleic acid. PKC inhibitor completely abolished oleic acid-induced NF-kappa B activation, suggesting that PKC activation is upstream of NF-kappa B activation in oleic acid-induced ET-1 expression. These data suggest that elevated plasma oleic acid levels observed in obese, insulin-resistant subjects result in endothelial dysfunction, at least in part, through an increase in ET-1 expression.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Endothelin-1,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Indoles,
http://linkedlifedata.com/resource/pubmed/chemical/Maleimides,
http://linkedlifedata.com/resource/pubmed/chemical/NF-kappa B,
http://linkedlifedata.com/resource/pubmed/chemical/Oleic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinase C,
http://linkedlifedata.com/resource/pubmed/chemical/Pyrrolidines,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Thiocarbamates,
http://linkedlifedata.com/resource/pubmed/chemical/bisindolylmaleimide I,
http://linkedlifedata.com/resource/pubmed/chemical/pyrrolidine dithiocarbamic acid
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0006-291X
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pubmed:author |
pubmed-author:KimMin SeonMS,
pubmed-author:KimYoung MiYM,
pubmed-author:KimYun MiYM,
pubmed-author:LeeIn KyuIK,
pubmed-author:LeeJae DamJD,
pubmed-author:LeeKi-UpKU,
pubmed-author:PakYoungmi KimYK,
pubmed-author:ParkJoong-YeolJY,
pubmed-author:ParkKi YoungKY,
pubmed-author:ParkSeung-JungSJ,
pubmed-author:SongHai SunHS
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pubmed:issnType |
Print
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pubmed:day |
11
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pubmed:volume |
303
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
891-5
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:12670494-Cells, Cultured,
pubmed-meshheading:12670494-Endothelin-1,
pubmed-meshheading:12670494-Endothelium, Vascular,
pubmed-meshheading:12670494-Enzyme Activation,
pubmed-meshheading:12670494-Enzyme Inhibitors,
pubmed-meshheading:12670494-Gene Expression,
pubmed-meshheading:12670494-Humans,
pubmed-meshheading:12670494-Indoles,
pubmed-meshheading:12670494-Insulin Resistance,
pubmed-meshheading:12670494-Maleimides,
pubmed-meshheading:12670494-NF-kappa B,
pubmed-meshheading:12670494-Obesity,
pubmed-meshheading:12670494-Oleic Acid,
pubmed-meshheading:12670494-Protein Kinase C,
pubmed-meshheading:12670494-Pyrrolidines,
pubmed-meshheading:12670494-RNA, Messenger,
pubmed-meshheading:12670494-Signal Transduction,
pubmed-meshheading:12670494-Thiocarbamates
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pubmed:year |
2003
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pubmed:articleTitle |
Oleic acid induces endothelin-1 expression through activation of protein kinase C and NF-kappa B.
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pubmed:affiliation |
Department of Internal Medicine, University of Ulsan College of Medicine, Poongnap-dong, Songpa-ku, 138-736, Seoul, South Korea
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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