Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2003-4-2
pubmed:abstractText
In previous studies, we demonstrated an over-expression of the dominant-negative isoform of the transcription factor Ikaros, Ik-6, in patients with B-cell malignancies, including blast crisis of chronic myelogenous leukaemia and acute lymphoblastic leukaemia. To investigate the consequence of over-expression of Ik-6 in B cells, we constructed Ik-6 transfectants of the FDH-1 and Ramos cell lines. FDH-1, which was established from a patient with early pre-B acute lymphoblastic leukaemia, undergoes apoptosis with dexamethasone treatment, whereas Ramos undergoes apoptosis following anti-IgM antibody treatment. Compared with the wild type, the over-expression of Ik-6 rendered the FDH-1 and Ramos transfectants resistant to dexamethasone-induced and anti-IgM-induced apoptosis respectively. An immunoblotting study demonstrated bcl-2 upregulation in anti-IgM-induced Ramos Ik-6 transfectants, but not in FDH-1 Ik-6 transfectants. Further investigations of the mechanism of leukaemogenesis associated with the over-expression of Ik-6 are warranted.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0007-1048
pubmed:author
pubmed:issnType
Print
pubmed:volume
121
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
165-9
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:12670348-Analysis of Variance, pubmed-meshheading:12670348-Antibodies, Anti-Idiotypic, pubmed-meshheading:12670348-Apoptosis, pubmed-meshheading:12670348-B-Lymphocytes, pubmed-meshheading:12670348-Burkitt Lymphoma, pubmed-meshheading:12670348-Cell Line, pubmed-meshheading:12670348-DNA-Binding Proteins, pubmed-meshheading:12670348-Dexamethasone, pubmed-meshheading:12670348-Flow Cytometry, pubmed-meshheading:12670348-Gene Expression, pubmed-meshheading:12670348-Glucocorticoids, pubmed-meshheading:12670348-Humans, pubmed-meshheading:12670348-Ikaros Transcription Factor, pubmed-meshheading:12670348-Immunoblotting, pubmed-meshheading:12670348-Immunoglobulin M, pubmed-meshheading:12670348-Precursor B-Cell Lymphoblastic Leukemia-Lymphoma, pubmed-meshheading:12670348-Protein Isoforms, pubmed-meshheading:12670348-Transcription Factors
pubmed:year
2003
pubmed:articleTitle
Over-expression of the dominant-negative isoform of Ikaros confers resistance to dexamethasone-induced and anti-IgM-induced apoptosis.
pubmed:affiliation
Department of Medicine, University of Okayama, 2-5-1 Shikata-cho, Okayama 700-8558, Japan.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't