Linked Life Data

12669881

Source:http://linkedlifedata.com/resource/pubmed/id/12669881

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2003-4-2
pubmed:abstractText
The increased release of prostaglandins (PG) within pulpal tissues is considered to play a pathogenic role during pulpal disease progression. The rate-limiting step in the formation of PG from arachidonic acid is catalyzed by cyclooxygenase (COX). COX-2 is an inducible enzyme believed to be responsible for PG synthesis at site of inflammation. The effect of proinflammatory cytokines on human pulp cells with special reference to COX-2 expression has not been reported earlier. The aim of the present study was to investigate the effects of interleukin (IL)-1alpha and tumor necrosis factor-alpha (TNF-alpha) on the expression of COX-2 mRNA gene and protein in cultured human pulp cells. Investigations of the time dependence of COX-2 mRNA expression in proinflammatory cytokines-treated human pulp cells revealed a rapid accumulation of the transcript, a significant signal first detectable 1 h after exposure. In addition, both IL-1alpha and TNF-alpha up-regulated COX-2 protein expression by human pulp cells. The kinetics of this response showed that COX-2 was detectable in cell lysates as early as 2 h post proinflammatory cytokines challenge and remained elevated throughout the 24-h incubation period. This suggests that one of the pathogenic mechanisms of pulpal inflammation in vivo may be the synthesis of COX-2 by resident cells in response to a proinflammatory cytokines challenge. COX-2 may play an important role in the regulation of prostanoid formation in the pathogenesis of pulpal inflammation. Taken together, we propose that the use of selective COX-2 inhibitors might provide a valuable tool in the control of pulpal inflammation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
D
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0099-2399
pubmed:author
pubmed:issnType
Print
pubmed:volume
29
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
201-4
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:12669881-Cell Culture Techniques, pubmed-meshheading:12669881-Cyclooxygenase 2, pubmed-meshheading:12669881-Dental Pulp, pubmed-meshheading:12669881-Enzyme Induction, pubmed-meshheading:12669881-Gene Expression Regulation, Enzymologic, pubmed-meshheading:12669881-Humans, pubmed-meshheading:12669881-Inflammation Mediators, pubmed-meshheading:12669881-Interleukin-1, pubmed-meshheading:12669881-Isoenzymes, pubmed-meshheading:12669881-Membrane Proteins, pubmed-meshheading:12669881-Peroxidases, pubmed-meshheading:12669881-Prostaglandin-Endoperoxide Synthases, pubmed-meshheading:12669881-Pulpitis, pubmed-meshheading:12669881-RNA, Messenger, pubmed-meshheading:12669881-Time Factors, pubmed-meshheading:12669881-Transcription, Genetic, pubmed-meshheading:12669881-Tumor Necrosis Factor-alpha, pubmed-meshheading:12669881-Up-Regulation
pubmed:year
2003
pubmed:articleTitle
Proinflammatory cytokines induce cyclooxygenase-2 mRNA and protein expression in human pulp cell cultures.
pubmed:affiliation
Institute of Stomatology, Chung Shan Medical University Hospital, Taichung, Taiwan.
pubmed:publicationType
Journal Article