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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
Pt 10
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pubmed:dateCreated |
2003-4-14
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pubmed:abstractText |
We examined the effect of the beta-subunits of the Na+/K+ and H+/K+ ATPases on the biogenesis of the sarcoplasmic reticulum (SR) Ca2+ ATPase in Xenopus oocytes. Oocytes were simultaneously injected with cRNAs for both the SR Ca2+ ATPase and the beta-subunit of the Na+/K+ or the H+/K+ ATPase. Immunoprecipitation with antiserum specific for the beta-subunit of the Na+/K+ or the H+/K+ ATPase yielded not only the respective beta-subunit but also the SR Ca2+ ATPase, indicating that the SR Ca2+ ATPase was associated with the beta-subunits of the Na+/K+ and the H+/K+ ATPases. Pulse-chase experiments revealed that the complex between the SR Ca2+ ATPase and the beta-subunit of the Na+/K+ ATPase was formed transiently and dissociated during the course of maturation. This is the first report that demonstrates the association of the SR Ca2+ ATPase with the beta-subunit of the Na+/K+ and H+/K+ ATPases.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0021-9533
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
116
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1875-80
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:12668725-Adenosine Triphosphatases,
pubmed-meshheading:12668725-Animals,
pubmed-meshheading:12668725-Blotting, Western,
pubmed-meshheading:12668725-Calcium,
pubmed-meshheading:12668725-Calcium-Transporting ATPases,
pubmed-meshheading:12668725-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:12668725-H(+)-K(+)-Exchanging ATPase,
pubmed-meshheading:12668725-Molecular Chaperones,
pubmed-meshheading:12668725-Oocytes,
pubmed-meshheading:12668725-Plasmids,
pubmed-meshheading:12668725-Precipitin Tests,
pubmed-meshheading:12668725-Protein Binding,
pubmed-meshheading:12668725-Protein Structure, Tertiary,
pubmed-meshheading:12668725-RNA, Complementary,
pubmed-meshheading:12668725-Sarcoplasmic Reticulum,
pubmed-meshheading:12668725-Sodium-Potassium-Exchanging ATPase,
pubmed-meshheading:12668725-Time Factors,
pubmed-meshheading:12668725-Trypsin,
pubmed-meshheading:12668725-Xenopus,
pubmed-meshheading:12668725-Xenopus laevis
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pubmed:year |
2003
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pubmed:articleTitle |
Transient association of the sarcoplasmic reticulum Ca2+ ATPase with the Na+/K+-ATPase and H+/K+-ATPase beta-subunits during its biogenesis in Xenopus oocytes.
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pubmed:affiliation |
Department of Biochemical Engineering and Science, Kyushu Institute of Technology Iizuka, Fukuoka 820-8502, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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