Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2003-4-1
pubmed:abstractText
Multiple dense phases of nucleosome core particles (NCPs) were formed in controlled ionic conditions (15-160 mM monovalent salt, no divalent ions), under osmotic pressures ranging from 4.7 x 10(5) to 2.35 x 10(6) Pa. We present here the x-ray diffraction analysis of these phases. In the lamello-columnar phase obtained at low salt concentration (<25 mM), NCPs stack into columns that align to form bilayers, kept separated from one another by a layer of solvent. NCPs form a monoclinic lattice in the plane of the bilayer. For high salt concentration (>50 mM), NCPs order into either a two-dimensional columnar hexagonal phase or into three-dimensional orthorhombic (quasi-hexagonal) crystals. The lamellar and hexagonal (or quasi-hexagonal) organizations coexist in the intermediate salt range; their demixing requires a long time. For an applied pressure P = 4.7 10(5) Pa, the calculated NCPs concentration ranges from approximately 280 to 320 mg/ml in the lamello-columnar phase to 495 to 585 mg/ml in the three-dimensional orthorhombic phase. These concentrations cover the concentration of the living cell.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/12668465-10777768, http://linkedlifedata.com/resource/pubmed/commentcorrection/12668465-11092917, http://linkedlifedata.com/resource/pubmed/commentcorrection/12668465-11566811, http://linkedlifedata.com/resource/pubmed/commentcorrection/12668465-11566884, http://linkedlifedata.com/resource/pubmed/commentcorrection/12668465-11736381, http://linkedlifedata.com/resource/pubmed/commentcorrection/12668465-11751321, http://linkedlifedata.com/resource/pubmed/commentcorrection/12668465-11882541, http://linkedlifedata.com/resource/pubmed/commentcorrection/12668465-12079350, http://linkedlifedata.com/resource/pubmed/commentcorrection/12668465-12097075, http://linkedlifedata.com/resource/pubmed/commentcorrection/12668465-1548703, http://linkedlifedata.com/resource/pubmed/commentcorrection/12668465-3790491, http://linkedlifedata.com/resource/pubmed/commentcorrection/12668465-4075395, http://linkedlifedata.com/resource/pubmed/commentcorrection/12668465-4092681, http://linkedlifedata.com/resource/pubmed/commentcorrection/12668465-559768, http://linkedlifedata.com/resource/pubmed/commentcorrection/12668465-6482966, http://linkedlifedata.com/resource/pubmed/commentcorrection/12668465-6682117, http://linkedlifedata.com/resource/pubmed/commentcorrection/12668465-6833394, http://linkedlifedata.com/resource/pubmed/commentcorrection/12668465-7265219, http://linkedlifedata.com/resource/pubmed/commentcorrection/12668465-9305837, http://linkedlifedata.com/resource/pubmed/commentcorrection/12668465-9336172, http://linkedlifedata.com/resource/pubmed/commentcorrection/12668465-9646870, http://linkedlifedata.com/resource/pubmed/commentcorrection/12668465-9811842
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0006-3495
pubmed:author
pubmed:issnType
Print
pubmed:volume
84
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2570-84
pubmed:dateRevised
2010-9-14
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
X-ray diffraction characterization of the dense phases formed by nucleosome core particles.
pubmed:affiliation
Laboratoire de Physique des Solides, Université Paris-Sud, 91405 Orsay Cédex, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't