Linked Life Data

12667927

Source:http://linkedlifedata.com/resource/pubmed/id/12667927

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2003-4-1
pubmed:abstractText
The aim of our study was to compare pharmacokinetic parameters of a highly bound protein drug, irbesartan, obtained from microdialysis data (MD) of arterial blood and conventional blood samples (BS). A new vascular shunt microdialysis probe was inserted into the carotid artery and one femoral vein was cannulated for i.v. administration of irbesartan. Microdialysis samples were collected every 15 min. Blood samples were taken every 15 min. Levels of drug were measured by HPLC. Pharmacokinetic parameters were estimated using TOPFIT program. Corrected MD were compared with BS taken at same time to determine protein binding. The irbesartan protein binding did not change during the experiment. The estimated Ke from MD and BS were similar (MD: 1.8+/-0.3 h(-1), n=5; BS: 1.7+/-0.2 h(-1), n=5). After protein binding correction for the MD, the estimated values of volume of distribution (Vd) (MD: 1.2+/-0.4 l, n=5; BS: 1.1+/-0.4 l, n=5), clearance (Cl) (MD: 32.3+/-7.3 ml min(-1), n=5; BS: 30.7+/-8.2 ml min(-1), n=5) and AUC (MD: 7.7+/-3.2 microg x ml(-1) h, n=5; BS: 8.8+/-3.4 microg x ml(-1) h, n=5) were similar between MD and BS. In conclusion, these results show that our new probe inserted in the carotid artery provides accurate MD to estimate pharmacokinetic parameters of a highly bound protein drug like irbesartan. On the other hand, MD were also useful to the in vivo study of drug protein binding and saturation in protein binding.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0731-7085
pubmed:author
pubmed:issnType
Print
pubmed:day
10
pubmed:volume
31
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1109-17
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
Validation of a new intraarterial microdialysis shunt probe for the estimation of pharmacokinetic parameters.
pubmed:affiliation
Cátedra de Farmacología, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956, (C1113AAD), Buenos Aires, Argentina. chocht@ffyb.uba.ar
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Validation Studies