Linked Life Data

12664141

Source:http://linkedlifedata.com/resource/pubmed/id/12664141

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2003-3-28
pubmed:abstractText
The biosynthesis of the aromatic polyene macrolide antibiotic candicidin, produced by Streptomyces griseus IMRU 3570, begins with a p-aminobenzoic acid (PABA) molecule which is activated to PABA-CoA and used as starter for the head-to-tail condensation of four propionate and 14 acetate units to produce a polyketide molecule to which the deoxysugar mycosamine is attached. Using the gene coding for the PABA synthase ( pabAB) from S. griseusIMRU 3570 as the probe, a 205-kb region of continuous DNA from the S. griseus chromosome was isolated and partially sequenced. Some of the genes possibly involved in the biosynthesis of candicidin were identified including part of the modular polyketide synthase (PKS), genes for thioesterase, deoxysugar biosynthesis, modification, transport, and regulatory proteins. The regulatory mechanisms involved in the production of candicidin, such as phosphate regulation, were studied using internal probes for some of the genes involved in the biosynthesis of the three moieties of candicidin (PKS, aromatic moiety and amino sugar). mRNAs specific for these genes were detected only in the production medium (SPG) but not in the SPG medium supplemented with phosphate or in the inoculum medium, indicating that phosphate represses the expression of genes involved in candicidin biosynthesis. The modular architecture of the candicidin PKS and the availability of the PKSs involved in the biosynthesis of three polyene antibiotics (pimaricin, nystatin, and amphotericin B) shall make possible the creation of new, less toxic and more active polyene antibiotics through combinatorial biosynthesis and targeted mutagenesis.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0175-7598
pubmed:author
pubmed:issnType
Print
pubmed:volume
60
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
633-42
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:12664141-4-Aminobenzoic Acid, pubmed-meshheading:12664141-Antifungal Agents, pubmed-meshheading:12664141-Bacterial Proteins, pubmed-meshheading:12664141-Candicidin, pubmed-meshheading:12664141-Carbon-Nitrogen Ligases, pubmed-meshheading:12664141-Chromosome Mapping, pubmed-meshheading:12664141-Chromosomes, Bacterial, pubmed-meshheading:12664141-Cloning, Molecular, pubmed-meshheading:12664141-Forecasting, pubmed-meshheading:12664141-Gene Expression Regulation, Bacterial, pubmed-meshheading:12664141-Genes, Bacterial, pubmed-meshheading:12664141-Molecular Structure, pubmed-meshheading:12664141-Open Reading Frames, pubmed-meshheading:12664141-Phosphates, pubmed-meshheading:12664141-RNA, Messenger, pubmed-meshheading:12664141-Streptomyces griseus, pubmed-meshheading:12664141-Transaminases
pubmed:year
2003
pubmed:articleTitle
Candicidin biosynthesis in Streptomyces griseus.
pubmed:affiliation
Departamento de Ecología, Genética y Microbiología, Area de Microbiología, Facultad de Ciencias Biológicas y Ambientales, Universidad de León, 24071 León, Spain. degjgs@unileon.es
pubmed:publicationType
Journal Article, Review, Research Support, Non-U.S. Gov't